Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum

Jun Shan Gao; Yan Meng; Nobumitsu Sasaki; Hiromi Kanegae; Naomi Hayashi; Hiroshi Nyunoya

doi:10.5897/ajb10.732

Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum

Jun Shan Gao^*, Yan Meng, Nobumitsu Sasaki, Hiromi Kanegae, Naomi Hayashi, Hiroshi Nyunoya

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.

Original language	English
Pages (from-to)	7998-8006
Number of pages	9
Journal	African Journal of Biotechnology
Volume	9
Issue number	47
DOIs	https://doi.org/10.5897/ajb10.732
Publication status	Published - 2010 Nov 22
Externally published	Yes

Keywords

Hypersensitive response
N gene
N homologue
Nicotiana tabacum
Tobacco mosaic virus (TMV)

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology
Molecular Biology
Genetics
Agronomy and Crop Science

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title = "Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum",

abstract = "Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.",

keywords = "Hypersensitive response, N gene, N homologue, Nicotiana tabacum, Tobacco mosaic virus (TMV)",

author = "Gao, {Jun Shan} and Yan Meng and Nobumitsu Sasaki and Hiromi Kanegae and Naomi Hayashi and Hiroshi Nyunoya",

year = "2010",

month = nov,

day = "22",

doi = "10.5897/ajb10.732",

language = "English",

volume = "9",

pages = "7998--8006",

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T1 - Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum

AU - Gao, Jun Shan

AU - Meng, Yan

AU - Sasaki, Nobumitsu

AU - Kanegae, Hiromi

AU - Hayashi, Naomi

AU - Nyunoya, Hiroshi

PY - 2010/11/22

Y1 - 2010/11/22

N2 - Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.

AB - Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.

KW - Hypersensitive response

KW - N gene

KW - N homologue

KW - Nicotiana tabacum

KW - Tobacco mosaic virus (TMV)

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Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum

Abstract

Keywords

ASJC Scopus subject areas

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