Characterization of a Deinococcus radiodurans MazF: A UACA-specific RNA endoribonuclease

Tatsuki Miyamoto; Yuri Ota; Akiko Yokota; Tetsushi Suyama; Satoshi Tsuneda; Naohiro Noda

doi:10.1002/mbo3.501

Characterization of a Deinococcus radiodurans MazF: A UACA-specific RNA endoribonuclease

Tatsuki Miyamoto, Yuri Ota, Akiko Yokota, Tetsushi Suyama, Satoshi Tsuneda^*, Naohiro Noda

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Microbes are known to withstand environmental stresses by using chromosomal toxin–antitoxin systems. MazEF is one of the most extensively studied toxin–antitoxin systems. In stressful environments, MazF toxins modulate translation by cleaving single-stranded RNAs in a sequence-specific fashion. Previously, a chromosomal gene located at DR0417 in Deinococcus radiodurans was predicted to code for a MazF endoribonuclease (MazF_DR ₀₄₁₇); however, its function remains unclear. In the present study, we characterized the molecular function of MazF_DR ₀₄₁₇. Analysis of MazF_DR ₀₄₁₇-cleaved RNA sites using modified massively parallel sequencing revealed a unique 4-nt motif, UACA, as a potential cleavage pattern. The activity of MazF_DR ₀₄₁₇ was also assessed in a real-time fluorometric assay, which revealed that MazF_DR ₀₄₁₇ strictly recognizes the unique tetrad UACA. This sequence specificity may allow D. radiodurans to alter its translation profile and survive under stressful conditions.

Original language	English
Article number	e00501
Journal	MicrobiologyOpen
Volume	6
Issue number	5
DOIs	https://doi.org/10.1002/mbo3.501
Publication status	Published - 2017 Oct

Keywords

Deinococcus radiodurans
MazEF
sequence specificity
toxin–antitoxin system

ASJC Scopus subject areas

Microbiology

Access to Document

10.1002/mbo3.501

Cite this

@article{792f70ffa7bb45489263a323300f8800,

title = "Characterization of a Deinococcus radiodurans MazF: A UACA-specific RNA endoribonuclease",

abstract = "Microbes are known to withstand environmental stresses by using chromosomal toxin–antitoxin systems. MazEF is one of the most extensively studied toxin–antitoxin systems. In stressful environments, MazF toxins modulate translation by cleaving single-stranded RNAs in a sequence-specific fashion. Previously, a chromosomal gene located at DR0417 in Deinococcus radiodurans was predicted to code for a MazF endoribonuclease (MazFDR 0417); however, its function remains unclear. In the present study, we characterized the molecular function of MazFDR 0417. Analysis of MazFDR 0417-cleaved RNA sites using modified massively parallel sequencing revealed a unique 4-nt motif, UACA, as a potential cleavage pattern. The activity of MazFDR 0417 was also assessed in a real-time fluorometric assay, which revealed that MazFDR 0417 strictly recognizes the unique tetrad UACA. This sequence specificity may allow D. radiodurans to alter its translation profile and survive under stressful conditions.",

keywords = "Deinococcus radiodurans, MazEF, sequence specificity, toxin–antitoxin system",

author = "Tatsuki Miyamoto and Yuri Ota and Akiko Yokota and Tetsushi Suyama and Satoshi Tsuneda and Naohiro Noda",

note = "Publisher Copyright: {\textcopyright} 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.",

year = "2017",

month = oct,

doi = "10.1002/mbo3.501",

language = "English",

volume = "6",

journal = "MicrobiologyOpen",

issn = "2045-8827",

publisher = "John Wiley & Sons Inc.",

number = "5",

}

TY - JOUR

T1 - Characterization of a Deinococcus radiodurans MazF

T2 - A UACA-specific RNA endoribonuclease

AU - Miyamoto, Tatsuki

AU - Ota, Yuri

AU - Yokota, Akiko

AU - Suyama, Tetsushi

AU - Tsuneda, Satoshi

AU - Noda, Naohiro

PY - 2017/10

Y1 - 2017/10

N2 - Microbes are known to withstand environmental stresses by using chromosomal toxin–antitoxin systems. MazEF is one of the most extensively studied toxin–antitoxin systems. In stressful environments, MazF toxins modulate translation by cleaving single-stranded RNAs in a sequence-specific fashion. Previously, a chromosomal gene located at DR0417 in Deinococcus radiodurans was predicted to code for a MazF endoribonuclease (MazFDR 0417); however, its function remains unclear. In the present study, we characterized the molecular function of MazFDR 0417. Analysis of MazFDR 0417-cleaved RNA sites using modified massively parallel sequencing revealed a unique 4-nt motif, UACA, as a potential cleavage pattern. The activity of MazFDR 0417 was also assessed in a real-time fluorometric assay, which revealed that MazFDR 0417 strictly recognizes the unique tetrad UACA. This sequence specificity may allow D. radiodurans to alter its translation profile and survive under stressful conditions.

AB - Microbes are known to withstand environmental stresses by using chromosomal toxin–antitoxin systems. MazEF is one of the most extensively studied toxin–antitoxin systems. In stressful environments, MazF toxins modulate translation by cleaving single-stranded RNAs in a sequence-specific fashion. Previously, a chromosomal gene located at DR0417 in Deinococcus radiodurans was predicted to code for a MazF endoribonuclease (MazFDR 0417); however, its function remains unclear. In the present study, we characterized the molecular function of MazFDR 0417. Analysis of MazFDR 0417-cleaved RNA sites using modified massively parallel sequencing revealed a unique 4-nt motif, UACA, as a potential cleavage pattern. The activity of MazFDR 0417 was also assessed in a real-time fluorometric assay, which revealed that MazFDR 0417 strictly recognizes the unique tetrad UACA. This sequence specificity may allow D. radiodurans to alter its translation profile and survive under stressful conditions.

KW - Deinococcus radiodurans

KW - MazEF

KW - sequence specificity

KW - toxin–antitoxin system

UR - http://www.scopus.com/inward/record.url?scp=85021717656&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021717656&partnerID=8YFLogxK

U2 - 10.1002/mbo3.501

DO - 10.1002/mbo3.501

M3 - Article

C2 - 28675659

AN - SCOPUS:85021717656

SN - 2045-8827

VL - 6

JO - MicrobiologyOpen

JF - MicrobiologyOpen

IS - 5

M1 - e00501

ER -

Characterization of a Deinococcus radiodurans MazF: A UACA-specific RNA endoribonuclease

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this