TY - JOUR
T1 - Characterization of a putative promoter region of the human poly(ADP-ribose) polymerase gene
T2 - Structural similarity to that of the DNA polymerase β gene
AU - Ogura, Tsutomu
AU - Nyunoya, Hiroshi
AU - Takahashi-Masutani, Mitsuko
AU - Miwa, Masanao
AU - Sugimura, Takashi
AU - Esumi, Hiroyasu
N1 - Funding Information:
ACKNOWLEDGMENTS: The authors thank Drs. Terada and K. Shimotohno for supplying the human cosmid genomic library and ~201 plasmid, respectively, and for valuable suggestions. They are also grateful to Drs. M. Nagao, Shima, N. Kato and T. Akagi for valuable discussion. work was supported in part by a Grant-in-Aid from the Ministry of Health and Welfare for the Comprehensive lo-year Strategy for Cancer Control, Japan, Grants in Aid for Cancer Research from the Ministry of Education, Science and Culture, and Haraguchi Memorial Cancer Research Fund. M. Takahashi-Masutani is a recipient of a Research Resident
PY - 1990/3/16
Y1 - 1990/3/16
N2 - The 5′-flanking region of the human poly(ADP-ribose) polymerase gene was isolated and characterized. The nucleotide sequence of a part of the poly(ADP-ribose) polymerase gene completely matched that of the cDNA. The transcriptional initiation sites (cap sites) of this gene, located about 166-bp upstream from the translational initiation site, were identified by S1 mapping analysis. Neither CAAT box nor TATA box was found within 500-bp upstream from the cap sites of poly(ADP-ribose) polymerase gene. The 200-bp immediately upstream of the cap site had a high G+C content (76.5%) and contained double repeats of the sequence CCGCCC, putative Sp1 binding sites, and a palindromic structure. The 5′-flanking region of poly(ADP-ribose) polymerase gene also showed promoter activity in chloramphenicol acetyltransferase assay and structural similarity to that of DNA polymerase β gene.
AB - The 5′-flanking region of the human poly(ADP-ribose) polymerase gene was isolated and characterized. The nucleotide sequence of a part of the poly(ADP-ribose) polymerase gene completely matched that of the cDNA. The transcriptional initiation sites (cap sites) of this gene, located about 166-bp upstream from the translational initiation site, were identified by S1 mapping analysis. Neither CAAT box nor TATA box was found within 500-bp upstream from the cap sites of poly(ADP-ribose) polymerase gene. The 200-bp immediately upstream of the cap site had a high G+C content (76.5%) and contained double repeats of the sequence CCGCCC, putative Sp1 binding sites, and a palindromic structure. The 5′-flanking region of poly(ADP-ribose) polymerase gene also showed promoter activity in chloramphenicol acetyltransferase assay and structural similarity to that of DNA polymerase β gene.
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U2 - 10.1016/0006-291X(90)92082-B
DO - 10.1016/0006-291X(90)92082-B
M3 - Article
C2 - 2108670
AN - SCOPUS:0025275438
SN - 0006-291X
VL - 167
SP - 701
EP - 710
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -