Characterization of amyloid β protein species in cerebral amyloid angiopathy of a squirrel monkey by immunocytochemistry and enzyme-linked immunosorbent assay

Naoya Sawamura; Akira Tamaoka; Shi N.Ichi Shoji; Edward H. Koo; Lary C. Walker; Hiroshi Mori

doi:10.1016/S0006-8993(97)00624-0

Characterization of amyloid β protein species in cerebral amyloid angiopathy of a squirrel monkey by immunocytochemistry and enzyme-linked immunosorbent assay

Naoya Sawamura, Akira Tamaoka^*, Shi N.Ichi Shoji, Edward H. Koo, Lary C. Walker, Hiroshi Mori

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

We analyzed the composition of amyloid β protein (A β) species in cerebral amyloid angiopathy (CAA) of an aged squirrel monkey. Immunocytochemistry demonstrated that the cerebral cortex contained no lesions other than widespread CAA with A β40 as its apparent major component. However, enzyme-linked immunosorbent assay revealed that A β42(43) predominated over A β40 in a formic acid-extracted cortical fraction. These findings suggest possible underestimation of A β42(43) levels in some previous immunocytochemical investigations.

Original language	English
Pages (from-to)	225-229
Number of pages	5
Journal	Brain Research
Volume	764
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(97)00624-0
Publication status	Published - 1997 Aug 1
Externally published	Yes

Keywords

Alzheimer's disease
Amyloid β protein
Cerebral amyloid angiopathy
Enzyme-linked immunosorbent assay
Immunocytochemistry
Squirrel monkey

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(97)00624-0

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title = "Characterization of amyloid β protein species in cerebral amyloid angiopathy of a squirrel monkey by immunocytochemistry and enzyme-linked immunosorbent assay",

abstract = "We analyzed the composition of amyloid β protein (A β) species in cerebral amyloid angiopathy (CAA) of an aged squirrel monkey. Immunocytochemistry demonstrated that the cerebral cortex contained no lesions other than widespread CAA with A β40 as its apparent major component. However, enzyme-linked immunosorbent assay revealed that A β42(43) predominated over A β40 in a formic acid-extracted cortical fraction. These findings suggest possible underestimation of A β42(43) levels in some previous immunocytochemical investigations.",

keywords = "Alzheimer's disease, Amyloid β protein, Cerebral amyloid angiopathy, Enzyme-linked immunosorbent assay, Immunocytochemistry, Squirrel monkey",

author = "Naoya Sawamura and Akira Tamaoka and Shoji, {Shi N.Ichi} and Koo, {Edward H.} and Walker, {Lary C.} and Hiroshi Mori",

note = "Funding Information: This work was in part supported by a Grant from the University of Tsukuba project research, by a Grant-in-Aid of the Japan Medical Association, by a Grant-in-Aid of the Sandoz Foundation for Gerontological Research, by a Grant-in-Aid of the ONO Medical Research Foundation, and by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, and for Health and Welfare of Japan to A.T.",

year = "1997",

month = aug,

day = "1",

doi = "10.1016/S0006-8993(97)00624-0",

language = "English",

volume = "764",

pages = "225--229",

journal = "Brain Research",

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TY - JOUR

T1 - Characterization of amyloid β protein species in cerebral amyloid angiopathy of a squirrel monkey by immunocytochemistry and enzyme-linked immunosorbent assay

AU - Sawamura, Naoya

AU - Tamaoka, Akira

AU - Shoji, Shi N.Ichi

AU - Koo, Edward H.

AU - Walker, Lary C.

AU - Mori, Hiroshi

N1 - Funding Information: This work was in part supported by a Grant from the University of Tsukuba project research, by a Grant-in-Aid of the Japan Medical Association, by a Grant-in-Aid of the Sandoz Foundation for Gerontological Research, by a Grant-in-Aid of the ONO Medical Research Foundation, and by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, and for Health and Welfare of Japan to A.T.

PY - 1997/8/1

Y1 - 1997/8/1

N2 - We analyzed the composition of amyloid β protein (A β) species in cerebral amyloid angiopathy (CAA) of an aged squirrel monkey. Immunocytochemistry demonstrated that the cerebral cortex contained no lesions other than widespread CAA with A β40 as its apparent major component. However, enzyme-linked immunosorbent assay revealed that A β42(43) predominated over A β40 in a formic acid-extracted cortical fraction. These findings suggest possible underestimation of A β42(43) levels in some previous immunocytochemical investigations.

AB - We analyzed the composition of amyloid β protein (A β) species in cerebral amyloid angiopathy (CAA) of an aged squirrel monkey. Immunocytochemistry demonstrated that the cerebral cortex contained no lesions other than widespread CAA with A β40 as its apparent major component. However, enzyme-linked immunosorbent assay revealed that A β42(43) predominated over A β40 in a formic acid-extracted cortical fraction. These findings suggest possible underestimation of A β42(43) levels in some previous immunocytochemical investigations.

KW - Alzheimer's disease

KW - Amyloid β protein

KW - Cerebral amyloid angiopathy

KW - Enzyme-linked immunosorbent assay

KW - Immunocytochemistry

KW - Squirrel monkey

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AN - SCOPUS:0030852418

SN - 0006-8993

VL - 764

SP - 225

EP - 229

JO - Brain Research

JF - Brain Research

IS - 1-2

ER -

Characterization of amyloid β protein species in cerebral amyloid angiopathy of a squirrel monkey by immunocytochemistry and enzyme-linked immunosorbent assay

Abstract

Keywords

ASJC Scopus subject areas

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