Characterization of extracellular RNAs produced by the marine photosynthetic bacterium Rhodovulum sulfidophilum

Tomoaki Ando; Hiromichi Suzuki; Seiichiro Nishimura; Terumichi Tanaka; Akira Hiraishi; Yo Kikuchi

doi:10.1093/jb/mvj091

Characterization of extracellular RNAs produced by the marine photosynthetic bacterium Rhodovulum sulfidophilum

Tomoaki Ando, Hiromichi Suzuki, Seiichiro Nishimura, Terumichi Tanaka, Akira Hiraishi, Yo Kikuchi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

The marine photosynthetic bacterium Rhodovulum sulfidophilum produces extracellular nucleic acids that are involved in its flocculation. These were found to be produced concomitantly with cell growth. The RNA fraction of these extracellular nucleic acids was subjected to cDNA analysis by applying a micro RNA cloning method and found to contain mainly fully mature-sized tRNAs and fragments of 16S and 23S rRNAs. Analyses of modified bases and genes of the RNAs revealed no structural difference between the intracellular and extracellular RNAs. This is the first report of structural analyses of bacterial extracellular RNAs.

Original language	English
Pages (from-to)	805-811
Number of pages	7
Journal	Journal of Biochemistry
Volume	139
Issue number	4
DOIs	https://doi.org/10.1093/jb/mvj091
Publication status	Published - 2006 Apr
Externally published	Yes

Keywords

Extracellular RNA
Flocculation
Photosynthetic bacterium
Rhodovulum sulfidophilum
RNA cloning

ASJC Scopus subject areas

Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/jb/mvj091

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title = "Characterization of extracellular RNAs produced by the marine photosynthetic bacterium Rhodovulum sulfidophilum",

abstract = "The marine photosynthetic bacterium Rhodovulum sulfidophilum produces extracellular nucleic acids that are involved in its flocculation. These were found to be produced concomitantly with cell growth. The RNA fraction of these extracellular nucleic acids was subjected to cDNA analysis by applying a micro RNA cloning method and found to contain mainly fully mature-sized tRNAs and fragments of 16S and 23S rRNAs. Analyses of modified bases and genes of the RNAs revealed no structural difference between the intracellular and extracellular RNAs. This is the first report of structural analyses of bacterial extracellular RNAs.",

keywords = "Extracellular RNA, Flocculation, Photosynthetic bacterium, Rhodovulum sulfidophilum, RNA cloning",

author = "Tomoaki Ando and Hiromichi Suzuki and Seiichiro Nishimura and Terumichi Tanaka and Akira Hiraishi and Yo Kikuchi",

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T1 - Characterization of extracellular RNAs produced by the marine photosynthetic bacterium Rhodovulum sulfidophilum

AU - Ando, Tomoaki

AU - Suzuki, Hiromichi

AU - Nishimura, Seiichiro

AU - Tanaka, Terumichi

AU - Hiraishi, Akira

AU - Kikuchi, Yo

PY - 2006/4

Y1 - 2006/4

N2 - The marine photosynthetic bacterium Rhodovulum sulfidophilum produces extracellular nucleic acids that are involved in its flocculation. These were found to be produced concomitantly with cell growth. The RNA fraction of these extracellular nucleic acids was subjected to cDNA analysis by applying a micro RNA cloning method and found to contain mainly fully mature-sized tRNAs and fragments of 16S and 23S rRNAs. Analyses of modified bases and genes of the RNAs revealed no structural difference between the intracellular and extracellular RNAs. This is the first report of structural analyses of bacterial extracellular RNAs.

AB - The marine photosynthetic bacterium Rhodovulum sulfidophilum produces extracellular nucleic acids that are involved in its flocculation. These were found to be produced concomitantly with cell growth. The RNA fraction of these extracellular nucleic acids was subjected to cDNA analysis by applying a micro RNA cloning method and found to contain mainly fully mature-sized tRNAs and fragments of 16S and 23S rRNAs. Analyses of modified bases and genes of the RNAs revealed no structural difference between the intracellular and extracellular RNAs. This is the first report of structural analyses of bacterial extracellular RNAs.

KW - Extracellular RNA

KW - Flocculation

KW - Photosynthetic bacterium

KW - Rhodovulum sulfidophilum

KW - RNA cloning

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JO - Journal of Biochemistry

JF - Journal of Biochemistry

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ER -

Characterization of extracellular RNAs produced by the marine photosynthetic bacterium Rhodovulum sulfidophilum

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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