Characterization of magnetic nanoparticles modified with thiol functionalized PAMAM dendron for DNA recovery

Tsuyoshi Tanaka; Keiyu Shibata; Masahito Hosokawa; Keiichi Hatakeyama; Atsushi Arakaki; Hideyuki Gomyo; Takeyuki Mogi; Tomoyuki Taguchi; Hitoshi Wake; Takeo Tanaami; Tadashi Matsunaga

doi:10.1016/j.jcis.2012.03.039

Characterization of magnetic nanoparticles modified with thiol functionalized PAMAM dendron for DNA recovery

Tsuyoshi Tanaka^*, Keiyu Shibata, Masahito Hosokawa, Keiichi Hatakeyama, Atsushi Arakaki, Hideyuki Gomyo, Takeyuki Mogi, Tomoyuki Taguchi, Hitoshi Wake, Takeo Tanaami, Tadashi Matsunaga

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Magnetic nanoparticles (MNPs) modified with the thiol functionalized polyamidoamine (PAMAM) dendron were synthesized to estimate their DNA recovery capabilities. Aminosilane-modified MNPs and MNPs surrounded by a phospholipid (distearoylphosphatidylethanolamine (DSPE)) bilayer were used as core particles. Cystamine-core PAMAM dendrimers were reduced by dithiothreitol to dendron thiols and chemically conjugated to the core particles. Characterization of the synthesis revealed an increase of the surface amine charge from generation 1 (G1) to G6, starting with an aminosilane initiator. Particle size distribution analysis indicated that G6 PAMAM-modified MNPs exhibited monodispersity in an aqueous solution. G6 PAMAM-MNPs and G6 PAMAM-PE-MNPs synthesized by the proposed method have equivalent DNA recovery abilities to PAMAM-MNPs prepared by the conventional divergent synthesis method. In optimized conditions, 96% of λDNA was recovered using G6 PAMAM-PE-MNPs. Therefore, the method for preparing PAMAM-MNPs and PAMAM-PE-MNPs proposed in this study will be a novel approach for producing DNA carriers for efficient DNA purification by magnetic separation.

Original language	English
Pages (from-to)	469-475
Number of pages	7
Journal	Journal of Colloid And Interface Science
Volume	377
Issue number	1
DOIs	https://doi.org/10.1016/j.jcis.2012.03.039
Publication status	Published - 2012 Jul 1
Externally published	Yes

Keywords

DNA carrier
Dendrimer
Magnetic nanoparticle
Phospholipid bilayer
Polyamidoamine

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Surfaces, Coatings and Films
Colloid and Surface Chemistry

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10.1016/j.jcis.2012.03.039

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title = "Characterization of magnetic nanoparticles modified with thiol functionalized PAMAM dendron for DNA recovery",

abstract = "Magnetic nanoparticles (MNPs) modified with the thiol functionalized polyamidoamine (PAMAM) dendron were synthesized to estimate their DNA recovery capabilities. Aminosilane-modified MNPs and MNPs surrounded by a phospholipid (distearoylphosphatidylethanolamine (DSPE)) bilayer were used as core particles. Cystamine-core PAMAM dendrimers were reduced by dithiothreitol to dendron thiols and chemically conjugated to the core particles. Characterization of the synthesis revealed an increase of the surface amine charge from generation 1 (G1) to G6, starting with an aminosilane initiator. Particle size distribution analysis indicated that G6 PAMAM-modified MNPs exhibited monodispersity in an aqueous solution. G6 PAMAM-MNPs and G6 PAMAM-PE-MNPs synthesized by the proposed method have equivalent DNA recovery abilities to PAMAM-MNPs prepared by the conventional divergent synthesis method. In optimized conditions, 96% of λDNA was recovered using G6 PAMAM-PE-MNPs. Therefore, the method for preparing PAMAM-MNPs and PAMAM-PE-MNPs proposed in this study will be a novel approach for producing DNA carriers for efficient DNA purification by magnetic separation.",

keywords = "DNA carrier, Dendrimer, Magnetic nanoparticle, Phospholipid bilayer, Polyamidoamine",

author = "Tsuyoshi Tanaka and Keiyu Shibata and Masahito Hosokawa and Keiichi Hatakeyama and Atsushi Arakaki and Hideyuki Gomyo and Takeyuki Mogi and Tomoyuki Taguchi and Hitoshi Wake and Takeo Tanaami and Tadashi Matsunaga",

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doi = "10.1016/j.jcis.2012.03.039",

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T1 - Characterization of magnetic nanoparticles modified with thiol functionalized PAMAM dendron for DNA recovery

AU - Tanaka, Tsuyoshi

AU - Shibata, Keiyu

AU - Hosokawa, Masahito

AU - Hatakeyama, Keiichi

AU - Arakaki, Atsushi

AU - Gomyo, Hideyuki

AU - Mogi, Takeyuki

AU - Taguchi, Tomoyuki

AU - Wake, Hitoshi

AU - Tanaami, Takeo

AU - Matsunaga, Tadashi

PY - 2012/7/1

Y1 - 2012/7/1

N2 - Magnetic nanoparticles (MNPs) modified with the thiol functionalized polyamidoamine (PAMAM) dendron were synthesized to estimate their DNA recovery capabilities. Aminosilane-modified MNPs and MNPs surrounded by a phospholipid (distearoylphosphatidylethanolamine (DSPE)) bilayer were used as core particles. Cystamine-core PAMAM dendrimers were reduced by dithiothreitol to dendron thiols and chemically conjugated to the core particles. Characterization of the synthesis revealed an increase of the surface amine charge from generation 1 (G1) to G6, starting with an aminosilane initiator. Particle size distribution analysis indicated that G6 PAMAM-modified MNPs exhibited monodispersity in an aqueous solution. G6 PAMAM-MNPs and G6 PAMAM-PE-MNPs synthesized by the proposed method have equivalent DNA recovery abilities to PAMAM-MNPs prepared by the conventional divergent synthesis method. In optimized conditions, 96% of λDNA was recovered using G6 PAMAM-PE-MNPs. Therefore, the method for preparing PAMAM-MNPs and PAMAM-PE-MNPs proposed in this study will be a novel approach for producing DNA carriers for efficient DNA purification by magnetic separation.

AB - Magnetic nanoparticles (MNPs) modified with the thiol functionalized polyamidoamine (PAMAM) dendron were synthesized to estimate their DNA recovery capabilities. Aminosilane-modified MNPs and MNPs surrounded by a phospholipid (distearoylphosphatidylethanolamine (DSPE)) bilayer were used as core particles. Cystamine-core PAMAM dendrimers were reduced by dithiothreitol to dendron thiols and chemically conjugated to the core particles. Characterization of the synthesis revealed an increase of the surface amine charge from generation 1 (G1) to G6, starting with an aminosilane initiator. Particle size distribution analysis indicated that G6 PAMAM-modified MNPs exhibited monodispersity in an aqueous solution. G6 PAMAM-MNPs and G6 PAMAM-PE-MNPs synthesized by the proposed method have equivalent DNA recovery abilities to PAMAM-MNPs prepared by the conventional divergent synthesis method. In optimized conditions, 96% of λDNA was recovered using G6 PAMAM-PE-MNPs. Therefore, the method for preparing PAMAM-MNPs and PAMAM-PE-MNPs proposed in this study will be a novel approach for producing DNA carriers for efficient DNA purification by magnetic separation.

KW - DNA carrier

KW - Dendrimer

KW - Magnetic nanoparticle

KW - Phospholipid bilayer

KW - Polyamidoamine

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Characterization of magnetic nanoparticles modified with thiol functionalized PAMAM dendron for DNA recovery

Abstract

Keywords

ASJC Scopus subject areas

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