Characterization of marine microalga, Scenedesmus sp. strain JPCC GA0024 toward biofuel production

Tadashi Matsunaga*, Mitsufumi Matsumoto, Yoshiaki Maeda, Hiroshi Sugiyama, Reiko Sato, Tsuyoshi Tanaka

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)


A marine microalga, strain JPCC GA0024 was selected as high amount of neutral lipid producers from marine microalgal culture collection toward biofuel production. The strain was tentatively identified as Scenedesmus rubescens by 18S rDNA analysis. The growth of strain JPCC GA0024 was influenced by artificial seawater concentrations. The optimum growth of 0.79 g/l was obtained at 100% artificial seawater. The lipid accumulation reached 73.0% of dry cell weight at 100% artificial seawater without additional nutrients for 11 days. Gas chromatography/mass spectrometry analysis indicates that lipid fraction mainly contained hydrocarbons including mainly hexadecane (C16 H34) and 1-docosene (C22 H44). Furthermore, calorimetric analysis revealed that the energy content of strain JPCC GA0024 was 6,160 kcal/kg (25.8 MJ/kg) of calorific value, which was equivalent to the coal engery. The strain JPCC GA0024, S.rubescens, will become a promising resource that can grow as a dominant species in the seawater for the production of both liquid and solid biofuels.

Original languageEnglish
Pages (from-to)1367-1372
Number of pages6
JournalBiotechnology Letters
Issue number9
Publication statusPublished - 2009 Aug
Externally publishedYes


  • Biofuels
  • Lipid
  • Marine microalgae
  • Scenedesmus rubescens

ASJC Scopus subject areas

  • Biotechnology


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