TY - JOUR
T1 - Characterization of reactive oxygen species generated by protoporphyrin IX under X-ray irradiation
AU - Takahashi, Junko
AU - Misawa, Masaki
N1 - Funding Information:
This work was supported by in part by a grant “Development of three-dimensional complex organ structures (2007–2010)” sponsored by New Energy and Industrial Technology Development Organization (NEDO).
PY - 2009/11
Y1 - 2009/11
N2 - As a possible radiosensitizer candidate having biological compatibility, oncotropic property, and X-ray activation capability, contribution of protoporphyrin IX (PpIX) to enhanced generation of reactive oxygen species (ROS) under X-ray and UV irradiations were examined. To identify the kinds of ROS, 2-[6-(4-amino)phenoxy-3H-xanthen-3-on-9-yl] benzoic acid (APF) and dihydroethidium (DHE) were used together with ethanol as a hydroxyl radical (OH{radical dot}) quencher. All of the three species of our interest (OH{radical dot}, superoxide radical (O2{radical dot}-), and singlet oxygen (1O2)) were enhanced by PpIX under X-ray and UV irradiations in addition to those by radiolysis and photolysis. Its enhancement factors exceeded 1.7 depending on the concentrations of PpIX from 1.5 to 15.0 μg/ml.
AB - As a possible radiosensitizer candidate having biological compatibility, oncotropic property, and X-ray activation capability, contribution of protoporphyrin IX (PpIX) to enhanced generation of reactive oxygen species (ROS) under X-ray and UV irradiations were examined. To identify the kinds of ROS, 2-[6-(4-amino)phenoxy-3H-xanthen-3-on-9-yl] benzoic acid (APF) and dihydroethidium (DHE) were used together with ethanol as a hydroxyl radical (OH{radical dot}) quencher. All of the three species of our interest (OH{radical dot}, superoxide radical (O2{radical dot}-), and singlet oxygen (1O2)) were enhanced by PpIX under X-ray and UV irradiations in addition to those by radiolysis and photolysis. Its enhancement factors exceeded 1.7 depending on the concentrations of PpIX from 1.5 to 15.0 μg/ml.
KW - Photodynamic therapy
KW - Photosensitizer
KW - Protoporphyrin
KW - Radiosensitizer
KW - Radiotherapy
KW - Reactive oxygen species
KW - X-ray
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U2 - 10.1016/j.radphyschem.2009.06.036
DO - 10.1016/j.radphyschem.2009.06.036
M3 - Article
AN - SCOPUS:68849090754
SN - 0969-806X
VL - 78
SP - 889
EP - 898
JO - Radiation Physics and Chemistry
JF - Radiation Physics and Chemistry
IS - 11
ER -