Characterizing and modulating the mechanical properties of hydrogels from ventricular extracellular matrix

Kyohei Fujita, Yuuki Tuchida, Hiroki Seki, Tadashi Kosawada, Zhonggang Feng, Yasuyuki Shiraishi, Daisuke Sato, Takao Nakamura, Mitsuo Umezu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)


In order to differentiate pluripotent stem cells to cardiomyocytes, the most general method is to expose stem cells to various growth factors related to cardiogenesis. However, a novel method has been reported to induce cardiac differentiation of human ES cells without supplemental growth factors by culturing embryoid body of human ES cells in hybrid gels composed of cardiac extracellular matrix (ECM) and type I collagen. On the other hand, mechanical properties of scaffold is one of the critical cue for differentiation of stem cells. However, it has not been thoroughly investigated the mechanical properties of the scaffold made from cardiac ECM in view of this and other reports about the differentiation of stem cells into cardiomyocytes using cardiac ECM scaffold. In this study, we fabricated bio-hydrogels composed of goat ventricular extracellular matrix, and investigated the mechanical properties by means of uniaxial compression test. It showed that the ECM gels possess viscoelastic property. The elastic modulus K1 in modified non-linear Kelvin model is 9.5 Pa for these gels and K2 is 814.7 Pa. Moreover, we were able to improve the elastic moduli K1 and K2 up to 139.7 Pa and 2023.9 Pa, respectively, by chemical treatment using EDAC.

Original languageEnglish
Title of host publication2015 10th Asian Control Conference
Subtitle of host publicationEmerging Control Techniques for a Sustainable World, ASCC 2015
EditorsHazlina Selamat, Hafiz Rashidi Haruna Ramli, Ahmad Athif Mohd Faudzi, Ribhan Zafira Abdul Rahman, Asnor Juraiza Ishak, Azura Che Soh, Siti Anom Ahmad
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781479978625
Publication statusPublished - 2015 Sept 8
Externally publishedYes
Event10th Asian Control Conference, ASCC 2015 - Kota Kinabalu, Malaysia
Duration: 2015 May 312015 Jun 3

Publication series

Name2015 10th Asian Control Conference: Emerging Control Techniques for a Sustainable World, ASCC 2015


Other10th Asian Control Conference, ASCC 2015
CityKota Kinabalu


  • cardiac extracellular matrix
  • hydrogel
  • viscoelasticity

ASJC Scopus subject areas

  • Control and Systems Engineering


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