Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex

Tadashi Matsunaga; Rika Sato; Shinji Kamiya; Tsuyosi Tanaka; Haruko Takeyama

doi:10.1016/S0304-8853(98)00575-7

Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex

Tadashi Matsunaga, Rika Sato, Shinji Kamiya, Tsuyosi Tanaka, Haruko Takeyama

Research output: Contribution to journal › Conference article › peer-review

69 Citations (Scopus)

Abstract

Bacterial magnetic particles (BMPs) which have ProteinA expressed on their surface were constructed using magA which is a key gene in BMP biosynthesis in the magnetic bacterium Magnetospirillum sp. AMB-1. Homogenous chemiluminescence enzyme immunoassay using antibody bound ProteinA-BMP complexes was developed for detection of human IgG. A good correlation between the luminescence yield and the concentration of human IgG was obtained in the range of 1-10³ ng/ml.

Original language	English
Pages (from-to)	126-131
Number of pages	6
Journal	Journal of Magnetism and Magnetic Materials
Volume	194
Issue number	1
DOIs	https://doi.org/10.1016/S0304-8853(98)00575-7
Publication status	Published - 1999 Apr
Externally published	Yes
Event	Proceedings of 1998 2nd International Conference on Scientific and Clinical Applications of Magnetic Carriers - Cleveland, OH, USA Duration: 1998 May 28 → 1998 May 30

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics

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title = "Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex",

abstract = "Bacterial magnetic particles (BMPs) which have ProteinA expressed on their surface were constructed using magA which is a key gene in BMP biosynthesis in the magnetic bacterium Magnetospirillum sp. AMB-1. Homogenous chemiluminescence enzyme immunoassay using antibody bound ProteinA-BMP complexes was developed for detection of human IgG. A good correlation between the luminescence yield and the concentration of human IgG was obtained in the range of 1-103 ng/ml.",

author = "Tadashi Matsunaga and Rika Sato and Shinji Kamiya and Tsuyosi Tanaka and Haruko Takeyama",

year = "1999",

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doi = "10.1016/S0304-8853(98)00575-7",

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journal = "Journal of Magnetism and Magnetic Materials",

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T1 - Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex

AU - Matsunaga, Tadashi

AU - Sato, Rika

AU - Kamiya, Shinji

AU - Tanaka, Tsuyosi

AU - Takeyama, Haruko

PY - 1999/4

Y1 - 1999/4

N2 - Bacterial magnetic particles (BMPs) which have ProteinA expressed on their surface were constructed using magA which is a key gene in BMP biosynthesis in the magnetic bacterium Magnetospirillum sp. AMB-1. Homogenous chemiluminescence enzyme immunoassay using antibody bound ProteinA-BMP complexes was developed for detection of human IgG. A good correlation between the luminescence yield and the concentration of human IgG was obtained in the range of 1-103 ng/ml.

AB - Bacterial magnetic particles (BMPs) which have ProteinA expressed on their surface were constructed using magA which is a key gene in BMP biosynthesis in the magnetic bacterium Magnetospirillum sp. AMB-1. Homogenous chemiluminescence enzyme immunoassay using antibody bound ProteinA-BMP complexes was developed for detection of human IgG. A good correlation between the luminescence yield and the concentration of human IgG was obtained in the range of 1-103 ng/ml.

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DO - 10.1016/S0304-8853(98)00575-7

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SN - 0304-8853

VL - 194

SP - 126

EP - 131

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

IS - 1

T2 - Proceedings of 1998 2nd International Conference on Scientific and Clinical Applications of Magnetic Carriers

Y2 - 28 May 1998 through 30 May 1998

ER -

Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex

Abstract

ASJC Scopus subject areas

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