Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles

Tadashi Matsunaga; Masashi Kawasaki; Xie Yu; Noriyuki Tsujimura; Noriyuki Nakamura

Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles

Tadashi Matsunaga^*, Masashi Kawasaki, Xie Yu, Noriyuki Tsujimura, Noriyuki Nakamura

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

113 Citations (Scopus)

Abstract

A novel chemiluminescence enzyme immunoassay using bacterial magnetic particles (BMPs) has been developed for highly sensitive and rapid detection of immunoglobulin G. Antibody was immobilized onto BMPs using the heterobifunctional reagents sulfosuccinimidyl 6-[3'-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) and sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1 -carboxylate (sulfo-SMCC). For the highly sensitive immunoassay method using these BMPs, a good relationship was obtained between the luminescence intensity and mouse IgG concentration in the range of 1-10⁵ fg/mL. Furthermore, in order to reduce assay time and to simplify operations, a rapid chemiluminescence enzyme immunoassay method has been developed. The rapid method was completed in 10 min. A linear relationship was obtained between the luminescence and mouse IgG concentration hi the range of 10-1000 ng/mL,.

Original language	English
Pages (from-to)	3551-3554
Number of pages	4
Journal	Analytical Chemistry
Volume	68
Issue number	20
Publication status	Published - 1996 Oct 15
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

Cite this

@article{4c193f4637e945f7a96e7c22492412a4,

title = "Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles",

abstract = "A novel chemiluminescence enzyme immunoassay using bacterial magnetic particles (BMPs) has been developed for highly sensitive and rapid detection of immunoglobulin G. Antibody was immobilized onto BMPs using the heterobifunctional reagents sulfosuccinimidyl 6-[3'-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) and sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1 -carboxylate (sulfo-SMCC). For the highly sensitive immunoassay method using these BMPs, a good relationship was obtained between the luminescence intensity and mouse IgG concentration in the range of 1-105 fg/mL. Furthermore, in order to reduce assay time and to simplify operations, a rapid chemiluminescence enzyme immunoassay method has been developed. The rapid method was completed in 10 min. A linear relationship was obtained between the luminescence and mouse IgG concentration hi the range of 10-1000 ng/mL,.",

author = "Tadashi Matsunaga and Masashi Kawasaki and Xie Yu and Noriyuki Tsujimura and Noriyuki Nakamura",

year = "1996",

month = oct,

day = "15",

language = "English",

volume = "68",

pages = "3551--3554",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "20",

}

TY - JOUR

T1 - Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles

AU - Matsunaga, Tadashi

AU - Kawasaki, Masashi

AU - Yu, Xie

AU - Tsujimura, Noriyuki

AU - Nakamura, Noriyuki

PY - 1996/10/15

Y1 - 1996/10/15

N2 - A novel chemiluminescence enzyme immunoassay using bacterial magnetic particles (BMPs) has been developed for highly sensitive and rapid detection of immunoglobulin G. Antibody was immobilized onto BMPs using the heterobifunctional reagents sulfosuccinimidyl 6-[3'-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) and sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1 -carboxylate (sulfo-SMCC). For the highly sensitive immunoassay method using these BMPs, a good relationship was obtained between the luminescence intensity and mouse IgG concentration in the range of 1-105 fg/mL. Furthermore, in order to reduce assay time and to simplify operations, a rapid chemiluminescence enzyme immunoassay method has been developed. The rapid method was completed in 10 min. A linear relationship was obtained between the luminescence and mouse IgG concentration hi the range of 10-1000 ng/mL,.

AB - A novel chemiluminescence enzyme immunoassay using bacterial magnetic particles (BMPs) has been developed for highly sensitive and rapid detection of immunoglobulin G. Antibody was immobilized onto BMPs using the heterobifunctional reagents sulfosuccinimidyl 6-[3'-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) and sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1 -carboxylate (sulfo-SMCC). For the highly sensitive immunoassay method using these BMPs, a good relationship was obtained between the luminescence intensity and mouse IgG concentration in the range of 1-105 fg/mL. Furthermore, in order to reduce assay time and to simplify operations, a rapid chemiluminescence enzyme immunoassay method has been developed. The rapid method was completed in 10 min. A linear relationship was obtained between the luminescence and mouse IgG concentration hi the range of 10-1000 ng/mL,.

UR - http://www.scopus.com/inward/record.url?scp=0030260484&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030260484&partnerID=8YFLogxK

M3 - Article

C2 - 8865763

AN - SCOPUS:0030260484

SN - 0003-2700

VL - 68

SP - 3551

EP - 3554

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 20

ER -

Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this