TY - JOUR
T1 - Cloning of bullfrog thyroid-stimulating hormone (TSH) β subunit cDNA
T2 - Expression of TSHβ mRNA during metamorphosis
AU - Okada, Reiko
AU - Iwata, Takeo
AU - Kato, Takafumi
AU - Kikuchi, Motoshi
AU - Yamamoto, Kazutoshi
AU - Kikuyama, Sakae
PY - 2000
Y1 - 2000
N2 - A thyroid-stimulating hormone β subunit (TSHβ) cDNA encoding both signal peptide and mature TSHβ molecule was cloned from a cDNA library constructed from total RNA of the bullfrog (Rana catesbeiana) adenohypophysis. The bullfrog TSHβ mRNA was estimated by Northern blot analysis to be approximately 1 kb. The deduced amino acid sequence showed 40-61% homologies with the sequences of TSH β subunits of other vertebrates. Using the cDNA as a probe, we measured changes in mRNA expression in metamorphosing tadpoles of R. catesbeiana. The TSH β subunit mRNA level increased progressively throughout prometamorphic stages, reaching its maximum at the end of prometamorphosis. The maximum level was maintained throughout early and mid climax, declining at late climax. These results, together with previously obtained data on plasma prolactin and pituitary prolactin mRNA levels, as well as thyroid hormone levels, are discussed in relation to metamorphic changes occurring in the bullfrog larvae. (C) 2000 Academic Press.
AB - A thyroid-stimulating hormone β subunit (TSHβ) cDNA encoding both signal peptide and mature TSHβ molecule was cloned from a cDNA library constructed from total RNA of the bullfrog (Rana catesbeiana) adenohypophysis. The bullfrog TSHβ mRNA was estimated by Northern blot analysis to be approximately 1 kb. The deduced amino acid sequence showed 40-61% homologies with the sequences of TSH β subunits of other vertebrates. Using the cDNA as a probe, we measured changes in mRNA expression in metamorphosing tadpoles of R. catesbeiana. The TSH β subunit mRNA level increased progressively throughout prometamorphic stages, reaching its maximum at the end of prometamorphosis. The maximum level was maintained throughout early and mid climax, declining at late climax. These results, together with previously obtained data on plasma prolactin and pituitary prolactin mRNA levels, as well as thyroid hormone levels, are discussed in relation to metamorphic changes occurring in the bullfrog larvae. (C) 2000 Academic Press.
KW - Bullfrog
KW - Metamorphosis
KW - TSHβ cDNA
KW - TSHβ mRNA expression
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U2 - 10.1006/gcen.2000.7515
DO - 10.1006/gcen.2000.7515
M3 - Article
C2 - 10936043
AN - SCOPUS:0033840892
SN - 0016-6480
VL - 119
SP - 224
EP - 231
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 2
ER -