TY - JOUR
T1 - CNTFR Genotype and Sprint/power Performance
T2 - Case-control Association and Functional Studies
AU - Miyamoto-Mikami, Eri
AU - Fujita, Y.
AU - Murakami, H.
AU - Ito, M.
AU - Miyachi, M.
AU - Kawahara, T.
AU - Fuku, N.
N1 - Funding Information:
This work was supported in part by grants from the programs Grants-in-Aid for Young Scientists (A-21680050 to N.F.) and Grants-in-Aid for Scientific Research from the Ministry of Health, Labor, and Welfare of Japan (to M.M.). E.M. was a recipient of a Grant-in-Aid for JSPS Fellows from the Japan Society for the Promotion of Science.
Publisher Copyright:
© Georg Thieme Verlag KG.
PY - 2016/2/2
Y1 - 2016/2/2
N2 - The aim of this study was to investigate whether rs41274853 in the 3′-untranslated region of the ciliary neurotrophic factor receptor gene (CNTFR) is associated with elite sprint/power athletic status and assess its functional significance. A total of 211 Japanese sprint/power track and field athletes (62 international, 72 national, and 77 regional athletes) and 814 Japanese controls were genotyped at rs41274853. Luciferase reporter assay was conducted to investigate whether this C-to-T polymorphism affects binding of microRNA miR-675-5p to this region. The TT genotype was significantly more frequent among international sprint/power athletes (19.4%) than in the controls after Bonferroni correction (7.9%, P=0.036, OR=2.81 [95% CI: 1.43-5.55]). Furthermore, in non-athletic young/middle-aged men (n=132), TT genotype carriers exhibited significantly greater leg extension power (26.6±5.4 vs. 24.0±5.4 W/kg BW, P=0.019) and vertical jump performance (50.1±6.9 vs. 47.9±7.5 cm, P=0.047) than the CC+CT genotype carriers. Reporter assays revealed that the miR-675-5p binds to this polymorphic region within the CNTFR mRNA, irrespective of the rs41274853 allele present. Although the functional significance of the rs41274853 polymorphism remains unclear, the CNTFR is one of the candidate genes contributing to sprint/power performance.
AB - The aim of this study was to investigate whether rs41274853 in the 3′-untranslated region of the ciliary neurotrophic factor receptor gene (CNTFR) is associated with elite sprint/power athletic status and assess its functional significance. A total of 211 Japanese sprint/power track and field athletes (62 international, 72 national, and 77 regional athletes) and 814 Japanese controls were genotyped at rs41274853. Luciferase reporter assay was conducted to investigate whether this C-to-T polymorphism affects binding of microRNA miR-675-5p to this region. The TT genotype was significantly more frequent among international sprint/power athletes (19.4%) than in the controls after Bonferroni correction (7.9%, P=0.036, OR=2.81 [95% CI: 1.43-5.55]). Furthermore, in non-athletic young/middle-aged men (n=132), TT genotype carriers exhibited significantly greater leg extension power (26.6±5.4 vs. 24.0±5.4 W/kg BW, P=0.019) and vertical jump performance (50.1±6.9 vs. 47.9±7.5 cm, P=0.047) than the CC+CT genotype carriers. Reporter assays revealed that the miR-675-5p binds to this polymorphic region within the CNTFR mRNA, irrespective of the rs41274853 allele present. Although the functional significance of the rs41274853 polymorphism remains unclear, the CNTFR is one of the candidate genes contributing to sprint/power performance.
KW - ciliary neurotrophic factor receptor
KW - elite sprint/power athletes
KW - single nucleotide polymorphism
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U2 - 10.1055/s-0035-1564257
DO - 10.1055/s-0035-1564257
M3 - Article
C2 - 26837930
AN - SCOPUS:84968718711
SN - 0172-4622
VL - 37
SP - 411
EP - 417
JO - International journal of sports medicine
JF - International journal of sports medicine
IS - 5
ER -