Coexpression of thrombopoietin and c-mpl genes in human acute myeloblastic leukemia cells

I. Matsumura, Y. Kanakura*, H. Ikeda, J. Ishikawa, H. Yoshida, Y. Horikawa, T. Nishiura, T. Tahara, T. Kato, H. Miyazaki, Y. Matsuzawa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


Thrombopoietin (TPO) is a recently identified hematopoietic growth factor that is essential for the growth and development of megakaryocytes. We have previously shown that TPO induces proliferation of acute myeloblastic leukemia (AML) cells in vitro. In this study, we have examined the expression of TPO and its receptor c-mpl in a series of AML cases and human leukemia cell lines. The mRNA transcripts of TPO were detectable in 18 of 50 AML cases and in some myeloid leukemia cell lines (HEL, M07E and CMK) by means of reverse transcriptase polymerase chain reaction (RT-PCR). In addition, TPO transcripts were coexpressed with c-mpl transcripts in 10 of 50 AML cases and in HEL, M07E and CMK cells. With regard to the French-American-British (FAB) classification, coexpression of TPO and c-mpl was observed with high frequency in AML cases of M7-type. Despite the TPO expression in a substantial fraction of leukemia cells, biological activity of TPO was not found in the conditioned medium that was obtained from cultivation of TPO mRNA-positive leukemia cells. These results suggest that TPO may not commonly participate in the abnormal growth of AML cells as an extracellular autocrine growth factor.

Original languageEnglish
Pages (from-to)91-94
Number of pages4
Issue number1
Publication statusPublished - 1996 Jan
Externally publishedYes


  • AML
  • Proliferation
  • Thrombopoietin
  • c-mpl

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research


Dive into the research topics of 'Coexpression of thrombopoietin and c-mpl genes in human acute myeloblastic leukemia cells'. Together they form a unique fingerprint.

Cite this