Comparison between the CENP-A and histone H3 structures in nucleosomes

Hiroaki Tachiwana; Wataru Kagawa; Hitoshi Kurumizaka

doi:10.4161/nucl.3.1.18372

Comparison between the CENP-A and histone H3 structures in nucleosomes

Hiroaki Tachiwana, Wataru Kagawa, Hitoshi Kurumizaka^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Centromeres are epigenetically marked by the assembly of nucleosomes containing the centromere-specific histone H3 variant, CENP-A (CENP-A nucleosome) and their inheritance is probably dictated by the architecture of the centromeric nucleosome. We previously determined the crystal structure of the human CENP-A nucleosome. CENP-A forms a histone octamer containing two each of histones H2A, H2B, H4 and CENP-A and the DNA is left-handedly wrapped around the histone octamer, as in canonical nucleosomes containing histone H3. In the CENP-A nucleosome structure, 13 base pairs of the DNA ends are detached from the histone surface and two CENP-A regions, the aN helix and loop 1, adopt different structures from those in the H3 nucleosome. In this Extra View article, we provide a detailed structural comparison between CENP-A and H3 in nucleosomes and describe their distinctions and similarities.

Original language	English
Pages (from-to)	6-11
Number of pages	6
Journal	Nucleus
Volume	3
Issue number	1
DOIs	https://doi.org/10.4161/nucl.3.1.18372
Publication status	Published - 2012 Jan

Keywords

CENP-A
CENP-B
CENP-C
Centromere
Chromatin
Histone
Histone H3
Nucleosome

ASJC Scopus subject areas

Cell Biology

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10.4161/nucl.3.1.18372

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title = "Comparison between the CENP-A and histone H3 structures in nucleosomes",

abstract = "Centromeres are epigenetically marked by the assembly of nucleosomes containing the centromere-specific histone H3 variant, CENP-A (CENP-A nucleosome) and their inheritance is probably dictated by the architecture of the centromeric nucleosome. We previously determined the crystal structure of the human CENP-A nucleosome. CENP-A forms a histone octamer containing two each of histones H2A, H2B, H4 and CENP-A and the DNA is left-handedly wrapped around the histone octamer, as in canonical nucleosomes containing histone H3. In the CENP-A nucleosome structure, 13 base pairs of the DNA ends are detached from the histone surface and two CENP-A regions, the aN helix and loop 1, adopt different structures from those in the H3 nucleosome. In this Extra View article, we provide a detailed structural comparison between CENP-A and H3 in nucleosomes and describe their distinctions and similarities.",

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author = "Hiroaki Tachiwana and Wataru Kagawa and Hitoshi Kurumizaka",

year = "2012",

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doi = "10.4161/nucl.3.1.18372",

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AU - Kagawa, Wataru

AU - Kurumizaka, Hitoshi

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Y1 - 2012/1

N2 - Centromeres are epigenetically marked by the assembly of nucleosomes containing the centromere-specific histone H3 variant, CENP-A (CENP-A nucleosome) and their inheritance is probably dictated by the architecture of the centromeric nucleosome. We previously determined the crystal structure of the human CENP-A nucleosome. CENP-A forms a histone octamer containing two each of histones H2A, H2B, H4 and CENP-A and the DNA is left-handedly wrapped around the histone octamer, as in canonical nucleosomes containing histone H3. In the CENP-A nucleosome structure, 13 base pairs of the DNA ends are detached from the histone surface and two CENP-A regions, the aN helix and loop 1, adopt different structures from those in the H3 nucleosome. In this Extra View article, we provide a detailed structural comparison between CENP-A and H3 in nucleosomes and describe their distinctions and similarities.

AB - Centromeres are epigenetically marked by the assembly of nucleosomes containing the centromere-specific histone H3 variant, CENP-A (CENP-A nucleosome) and their inheritance is probably dictated by the architecture of the centromeric nucleosome. We previously determined the crystal structure of the human CENP-A nucleosome. CENP-A forms a histone octamer containing two each of histones H2A, H2B, H4 and CENP-A and the DNA is left-handedly wrapped around the histone octamer, as in canonical nucleosomes containing histone H3. In the CENP-A nucleosome structure, 13 base pairs of the DNA ends are detached from the histone surface and two CENP-A regions, the aN helix and loop 1, adopt different structures from those in the H3 nucleosome. In this Extra View article, we provide a detailed structural comparison between CENP-A and H3 in nucleosomes and describe their distinctions and similarities.

KW - CENP-A

KW - CENP-B

KW - CENP-C

KW - Centromere

KW - Chromatin

KW - Histone

KW - Histone H3

KW - Nucleosome

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Comparison between the CENP-A and histone H3 structures in nucleosomes

Abstract

Keywords

ASJC Scopus subject areas

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