TY - JOUR
T1 - Complete nucleotide sequence of pLD-TEX-KL, a 66-kb plasmid of Legionella dumoffii TEX-KL strain
AU - Qin, Tian
AU - Hirakawa, Hideki
AU - Iida, Ken ichiro
AU - Oshima, Kenshiro
AU - Hattori, Masahira
AU - Tashiro, Kosuke
AU - Kuhara, Satoru
AU - Yoshida, Shin ichi
PY - 2007/11
Y1 - 2007/11
N2 - The complete nucleotide sequence of a large (66 kb) plasmid pLD-TEX-KL of Legionella dumoffii TEX-KL strain was determined. Of the 57 predicted open reading frames (ORFs), 39 (68%) encoded proteins similar to previously known proteins, five (9%) were assigned with putative functions, three (5%) encoded conserved hypothetical proteins, and 10 (18%) had no homology to any genes present in the current open databases. The ORFs with similar functions were organized in a modular structure; thus, transfer region was identified, as well as a putative heavy-metal ion transporter system (hel). The transfer region encoded homologs of the Salmonella entrica serovar Typhi conjugative system components involved in conjugation. In addition, we also found a potential protein that was analogous to the DNA polymerase III epsilon subunit. It is rarely found that plasmid encode the DNA polymerase.
AB - The complete nucleotide sequence of a large (66 kb) plasmid pLD-TEX-KL of Legionella dumoffii TEX-KL strain was determined. Of the 57 predicted open reading frames (ORFs), 39 (68%) encoded proteins similar to previously known proteins, five (9%) were assigned with putative functions, three (5%) encoded conserved hypothetical proteins, and 10 (18%) had no homology to any genes present in the current open databases. The ORFs with similar functions were organized in a modular structure; thus, transfer region was identified, as well as a putative heavy-metal ion transporter system (hel). The transfer region encoded homologs of the Salmonella entrica serovar Typhi conjugative system components involved in conjugation. In addition, we also found a potential protein that was analogous to the DNA polymerase III epsilon subunit. It is rarely found that plasmid encode the DNA polymerase.
KW - Complete nucleotide sequence
KW - Legionella dumoffii
KW - Plasmid
UR - http://www.scopus.com/inward/record.url?scp=35348955457&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35348955457&partnerID=8YFLogxK
U2 - 10.1016/j.plasmid.2007.08.001
DO - 10.1016/j.plasmid.2007.08.001
M3 - Article
C2 - 17881053
AN - SCOPUS:35348955457
SN - 0147-619X
VL - 58
SP - 261
EP - 268
JO - Plasmid
JF - Plasmid
IS - 3
ER -