TY - JOUR
T1 - Comprehensive single-cell transcriptome analysis reveals heterogeneity in endometrioid adenocarcinoma tissues
AU - Hashimoto, Shinichi
AU - Tabuchi, Yuta
AU - Yurino, Hideaki
AU - Hirohashi, Yoshihiko
AU - Deshimaru, Shungo
AU - Asano, Takuya
AU - Mariya, Tasuku
AU - Oshima, Kenshiro
AU - Takamura, Yuzuru
AU - Ukita, Yoshiaki
AU - Ametani, Akio
AU - Kondo, Naoto
AU - Monma, Norikazu
AU - Takeda, Tadayuki
AU - Misu, Sadahiko
AU - Okayama, Toshitugu
AU - Ikeo, Kazuho
AU - Saito, Tsuyoshi
AU - Kaneko, Shuich
AU - Suzuki, Yutaka
AU - Hattori, Masahira
AU - Matsushima, Kouji
AU - Torigoe, Toshihiko
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Single cell transcriptome analysis of a cancer tissue can provide objective assessment of subtype population or the activation of each of various microenvironment component cells. In this study, we applied our newly developed technique of single cell analysis to the myometrial infiltration side (M-side) and the endometrial side (E-side) of a human endometrioid adenocarcinoma with squamous differentiation tissues. We also analyzed spherogenic cultures derived from the same tissue to identify putative regulators of stemness in vivo. Cancer cells in the E-side were highly malignant compared with those in the M-side. Many cells on the E-side were positive for spheroid-specific tumorigenesis-related markers including SOX2. In addition, there were higher numbers of epithelial-to-mesenchymal transition (EMT) cells in the E-side compared with the M-side. This study identified a site containing cells with high malignant potential such as EMT and cancer stem-like cells in cancer tissues. Finally, we demonstrate that established endometrioid adenocarcinoma subtype classifiers were variably expressed across individual cells within a tumor. Thus, such intratumoral heterogeneity may be related to prognostic implications.
AB - Single cell transcriptome analysis of a cancer tissue can provide objective assessment of subtype population or the activation of each of various microenvironment component cells. In this study, we applied our newly developed technique of single cell analysis to the myometrial infiltration side (M-side) and the endometrial side (E-side) of a human endometrioid adenocarcinoma with squamous differentiation tissues. We also analyzed spherogenic cultures derived from the same tissue to identify putative regulators of stemness in vivo. Cancer cells in the E-side were highly malignant compared with those in the M-side. Many cells on the E-side were positive for spheroid-specific tumorigenesis-related markers including SOX2. In addition, there were higher numbers of epithelial-to-mesenchymal transition (EMT) cells in the E-side compared with the M-side. This study identified a site containing cells with high malignant potential such as EMT and cancer stem-like cells in cancer tissues. Finally, we demonstrate that established endometrioid adenocarcinoma subtype classifiers were variably expressed across individual cells within a tumor. Thus, such intratumoral heterogeneity may be related to prognostic implications.
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U2 - 10.1038/s41598-017-14676-3
DO - 10.1038/s41598-017-14676-3
M3 - Article
C2 - 29079795
AN - SCOPUS:85032435029
SN - 2045-2322
VL - 7
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 14225
ER -
