Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry

Nanako Azegami; Kazumi Saikusa; Yasuto Todokoro; Aritaka Nagadoi; Hitoshi Kurumizaka; Yoshifumi Nishimura; Satoko Akashi

doi:10.1021/bi4005655

Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry

Nanako Azegami, Kazumi Saikusa, Yasuto Todokoro, Aritaka Nagadoi, Hitoshi Kurumizaka, Yoshifumi Nishimura^*, Satoko Akashi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

It has been suggested that the hexasome, in which one of the H2A/H2B dimers is depleted from the canonical nucleosome core particle (NCP), is an essential intermediate during NCP assembly and disassembly, but little structural evidence of this exists. In this study, reconstituted products in a conventional NCP preparation were analyzed by native electrospray ionization mass spectrometry, and it was found that the hexasome, which migrated in a manner almost identical to that of the octasome NCP in native polyacrylamide gel electrophoresis, was produced simultaneously with the octasome NCP. This result might contribute to understanding the assembly and disassembly mechanism of NCPs.

Original language	English
Pages (from-to)	5155-5157
Number of pages	3
Journal	Biochemistry
Volume	52
Issue number	31
DOIs	https://doi.org/10.1021/bi4005655
Publication status	Published - 2013 Aug 6

ASJC Scopus subject areas

Biochemistry

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AU - Azegami, Nanako

AU - Saikusa, Kazumi

AU - Todokoro, Yasuto

AU - Nagadoi, Aritaka

AU - Kurumizaka, Hitoshi

AU - Nishimura, Yoshifumi

AU - Akashi, Satoko

PY - 2013/8/6

Y1 - 2013/8/6

N2 - It has been suggested that the hexasome, in which one of the H2A/H2B dimers is depleted from the canonical nucleosome core particle (NCP), is an essential intermediate during NCP assembly and disassembly, but little structural evidence of this exists. In this study, reconstituted products in a conventional NCP preparation were analyzed by native electrospray ionization mass spectrometry, and it was found that the hexasome, which migrated in a manner almost identical to that of the octasome NCP in native polyacrylamide gel electrophoresis, was produced simultaneously with the octasome NCP. This result might contribute to understanding the assembly and disassembly mechanism of NCPs.

AB - It has been suggested that the hexasome, in which one of the H2A/H2B dimers is depleted from the canonical nucleosome core particle (NCP), is an essential intermediate during NCP assembly and disassembly, but little structural evidence of this exists. In this study, reconstituted products in a conventional NCP preparation were analyzed by native electrospray ionization mass spectrometry, and it was found that the hexasome, which migrated in a manner almost identical to that of the octasome NCP in native polyacrylamide gel electrophoresis, was produced simultaneously with the octasome NCP. This result might contribute to understanding the assembly and disassembly mechanism of NCPs.

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Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry

Abstract

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