Condensin-mediated remodeling of the mitotic chromatin landscape in fission yeast

Yasutaka Kakui; Adam Rabinowitz; David J. Barry; Frank Uhlmann

doi:10.1038/ng.3938

Condensin-mediated remodeling of the mitotic chromatin landscape in fission yeast

Yasutaka Kakui, Adam Rabinowitz, David J. Barry, Frank Uhlmann^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

The eukaryotic genome consists of DNA molecules far longer than the cells that contain them. They reach their greatest compaction during chromosome condensation in mitosis. This process is aided by condensin, a structural maintenance of chromosomes (SMC) family member1,2. The spatial organization of mitotic chromosomes and how condensin shapes chromatin architecture are not yet fully understood. Here we use chromosome conformation capture (Hi-C)3,4 to study mitotic chromosome condensation in the fission yeast Schizosaccharomyces pombe5-7. This showed that the interphase landscape characterized by small chromatin domains is replaced by fewer but larger domains in mitosis. Condensin achieves this by setting up longer-range, intrachromosomal DNA interactions, which compact and individualize chromosomes. At the same time, local chromatin contacts are constrained by condensin, with profound implications for local chromatin function during mitosis. Our results highlight condensin as a major determinant that changes the chromatin landscape as cells prepare their genomes for cell division.

Original language	English
Pages (from-to)	1553-1557
Number of pages	5
Journal	Nature Genetics
Volume	49
Issue number	10
DOIs	https://doi.org/10.1038/ng.3938
Publication status	Published - 2017 Oct 1
Externally published	Yes

ASJC Scopus subject areas

Genetics

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10.1038/ng.3938

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title = "Condensin-mediated remodeling of the mitotic chromatin landscape in fission yeast",

abstract = "The eukaryotic genome consists of DNA molecules far longer than the cells that contain them. They reach their greatest compaction during chromosome condensation in mitosis. This process is aided by condensin, a structural maintenance of chromosomes (SMC) family member1,2. The spatial organization of mitotic chromosomes and how condensin shapes chromatin architecture are not yet fully understood. Here we use chromosome conformation capture (Hi-C)3,4 to study mitotic chromosome condensation in the fission yeast Schizosaccharomyces pombe5-7. This showed that the interphase landscape characterized by small chromatin domains is replaced by fewer but larger domains in mitosis. Condensin achieves this by setting up longer-range, intrachromosomal DNA interactions, which compact and individualize chromosomes. At the same time, local chromatin contacts are constrained by condensin, with profound implications for local chromatin function during mitosis. Our results highlight condensin as a major determinant that changes the chromatin landscape as cells prepare their genomes for cell division.",

author = "Yasutaka Kakui and Adam Rabinowitz and Barry, {David J.} and Frank Uhlmann",

note = "Funding Information: We would like to thank J. Abella and M. Way for their help with high-speed microscopy, A. Stewart for bioinformatic support, C. Haering (EMBL, Heidelberg) for Slp1 shutoff and chromosomal loci–tagged strains and H. Masukata (Osaka University) for the Skp1–Tir1 strain and AID plasmids, and P. Bates, E. Wershof, B. Khatri, Y. Murayama, T. Toda and our laboratory members for discussions and critical reading of the manuscript. This work was supported by the European Research Council and the Francis Crick Institute, which receives its core funding from Cancer Research UK (FC001198), the UK Medical Research Council (FC001198) and the Wellcome Trust (FC001198). Y.K. was supported by the Japanese Society for the Promotion of Science (JSPS Overseas Research Fellowships). Publisher Copyright: {\textcopyright} 2017 Nature America, Inc., part of Springer Nature. All rights reserved.",

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N2 - The eukaryotic genome consists of DNA molecules far longer than the cells that contain them. They reach their greatest compaction during chromosome condensation in mitosis. This process is aided by condensin, a structural maintenance of chromosomes (SMC) family member1,2. The spatial organization of mitotic chromosomes and how condensin shapes chromatin architecture are not yet fully understood. Here we use chromosome conformation capture (Hi-C)3,4 to study mitotic chromosome condensation in the fission yeast Schizosaccharomyces pombe5-7. This showed that the interphase landscape characterized by small chromatin domains is replaced by fewer but larger domains in mitosis. Condensin achieves this by setting up longer-range, intrachromosomal DNA interactions, which compact and individualize chromosomes. At the same time, local chromatin contacts are constrained by condensin, with profound implications for local chromatin function during mitosis. Our results highlight condensin as a major determinant that changes the chromatin landscape as cells prepare their genomes for cell division.

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Condensin-mediated remodeling of the mitotic chromatin landscape in fission yeast

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