TY - JOUR
T1 - Conformational properties of a novel modified nucleoside, 5-formylcytidine, found at the first position of the anticodon of bovine mitochondrial trnamet
AU - Watanabe, Kimitsuna
AU - Kawai, Gota
AU - Ueda, Takuya
N1 - Funding Information:
ACKNOWLEDGMENT This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas 04272102 from the Ministry of Education, Science and Culture of Japan, and grants from Human Frontier Science Program Organization, and the National Institute of General Medical Sciences (GM29812).
PY - 1994/6/1
Y1 - 1994/6/1
N2 - formylcytidine (f^C), which is found at the first position of the anticodon of bovine mitochondrial tRNA^e^, were analyzed by ^H-NMR spectroscopy. f^C has a normal amino tautomeric form at position 4 of the base moiety. The results indicate the presence of an intramolecular hydrogen bond between the carbonyl of the 5-formyl group and the 4-amino function. f 5 C was found to exhibit the C3’-endo conformation exclusively and the enthalpy difference (AH) between the C2’-endo and C3’-endo forms was found to be 1.56 ± 0.13 kcal/mol, indicating f5C to be one of the most conformationally rigid nucleosides yet analyzed. The conformational rigidity of f C may contribute to regulation of codon recognition by tRNAMet. Conformational properties of a novel modified nucleoside, 5-.
AB - formylcytidine (f^C), which is found at the first position of the anticodon of bovine mitochondrial tRNA^e^, were analyzed by ^H-NMR spectroscopy. f^C has a normal amino tautomeric form at position 4 of the base moiety. The results indicate the presence of an intramolecular hydrogen bond between the carbonyl of the 5-formyl group and the 4-amino function. f 5 C was found to exhibit the C3’-endo conformation exclusively and the enthalpy difference (AH) between the C2’-endo and C3’-endo forms was found to be 1.56 ± 0.13 kcal/mol, indicating f5C to be one of the most conformationally rigid nucleosides yet analyzed. The conformational rigidity of f C may contribute to regulation of codon recognition by tRNAMet. Conformational properties of a novel modified nucleoside, 5-.
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U2 - 10.1080/15257779408011889
DO - 10.1080/15257779408011889
M3 - Article
AN - SCOPUS:0028358136
SN - 0732-8311
VL - 13
SP - 1189
EP - 1199
JO - Nucleosides and Nucleotides
JF - Nucleosides and Nucleotides
IS - 5
ER -