TY - JOUR
T1 - Constitutive production of aconitate isomerase by Pseudomonas sp. WU-0701 in relation to trans-aconitic acid assimilation
AU - Takiguchi, Arisa
AU - Yoshioka, Isato
AU - Oda, Yunosuke
AU - Ishii, Yoshitaka
AU - Kirimura, Kohtaro
N1 - Funding Information:
We thank Dr. Masanori Suzuki for a helpful discussion on this work. This work was partially supported by Grants-in-Aid for Scientific Research, KAKENHI ( 20H02906 ), from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, and this paper is a part of the outcome of research performed under a Waseda University Grant for Special Research Projects ( 2019R-036 ) from Waseda University , Japan.
Publisher Copyright:
© 2020 The Society for Biotechnology, Japan
PY - 2021/1
Y1 - 2021/1
N2 - Aconitic acid, an unsaturated tricarboxylic acid, is used in the chemical industry as raw materials for organic synthesis, especially as a specific substrate for a flavoring agent. trans-Aconitic acid (tAA) is a trans-isomer of cis-aconitic acid and detected in some plants and bacteria. However, biosynthetic route and metabolism of tAA in relation to assimilation have been unknown. Aconitate isomerase (AI; EC 5.3.3.7) catalyzes the reversible isomerization between cis-aconitic acid and tAA. Pseudomonas sp. WU-0701 was isolated as a bacterium assimilating tAA as sole carbon source, and characterization and gene identification of AI were already reported. Here, we describe that Pseudomonas sp. WU-0701 exhibited growth in each synthetic medium containing glucose, citric acid, isocitric acid, or tAA as sole carbon source. AI was intracellularly detected all the time during the cultivation of the strain WU-0701 cells, irrespective of the carbon sources; AI activity was detected even in the glucose-grown cells. Through the subcellular fractionation experiments, AI was detected in the periplasmic fraction. This is the first report indicating that a bacterium belonging to the genus Pseudomonas is constitutive for the AI production.
AB - Aconitic acid, an unsaturated tricarboxylic acid, is used in the chemical industry as raw materials for organic synthesis, especially as a specific substrate for a flavoring agent. trans-Aconitic acid (tAA) is a trans-isomer of cis-aconitic acid and detected in some plants and bacteria. However, biosynthetic route and metabolism of tAA in relation to assimilation have been unknown. Aconitate isomerase (AI; EC 5.3.3.7) catalyzes the reversible isomerization between cis-aconitic acid and tAA. Pseudomonas sp. WU-0701 was isolated as a bacterium assimilating tAA as sole carbon source, and characterization and gene identification of AI were already reported. Here, we describe that Pseudomonas sp. WU-0701 exhibited growth in each synthetic medium containing glucose, citric acid, isocitric acid, or tAA as sole carbon source. AI was intracellularly detected all the time during the cultivation of the strain WU-0701 cells, irrespective of the carbon sources; AI activity was detected even in the glucose-grown cells. Through the subcellular fractionation experiments, AI was detected in the periplasmic fraction. This is the first report indicating that a bacterium belonging to the genus Pseudomonas is constitutive for the AI production.
KW - Aconitate isomerase
KW - Carbon catabolite repression
KW - Constitutive production
KW - Pseudomonas
KW - trans-Aconitic acid
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U2 - 10.1016/j.jbiosc.2020.09.003
DO - 10.1016/j.jbiosc.2020.09.003
M3 - Article
C2 - 32994133
AN - SCOPUS:85091686004
SN - 1389-1723
VL - 131
SP - 47
EP - 52
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 1
ER -