TY - JOUR
T1 - Construction of artificial methemoglobin reduction systems in Hb vesicles
AU - Takeoka, Shinji
AU - Ohgushi, Tekeru
AU - Sakai, Hiromi
AU - Kose, Takehiro
AU - Nishide, Hiroyuki
AU - Tsuchida, Eishun
N1 - Funding Information:
This work was supported in part by Grants-in-Aid from the Ministry of Education, Science, and Culture, Japan (No. 0651 59,07508005, and 08680940) and the Kawakami Memorial Foundation. The authors thank to Drs. Y. Izumi, A. Yoshizu, and K. Kobayashi (Department of Surgery, Keio University) for their technical cooperation in animal tests.
PY - 1997
Y1 - 1997
N2 - The hemoglobin vesicle (HbV) is a red cell substitute encapsulating purified concentrated Hb in a phospholipid vesicle. In order to suppress metHb formation for the long term maintenance of oxygen transporting capability in vivo, thiols (cysteine, Cys; homocysteine, Hcy) were studied as reductants of metHb. Hcy showed a suppressive effect on metHb formation, while Cys adversely accelerates metHb formation at the rate of twice the Hb solution without any reductants. The suppression of Cys-induced metHb formation by the addition of superoxide dismutase (SOD) and catalase indicated that Cys was easily oxidized by oxygen and simultaneously generated a large amount of active oxygens. The rate of metHb formation was influenced by PO2 and pH. Furthermore, the reducing systems (methylene blue (MB), NADH or ascorbic acid) were added to the outer aqueous phase of HbV, and the artificial reduction systems constructed through the bilayer membrane were evaluated.
AB - The hemoglobin vesicle (HbV) is a red cell substitute encapsulating purified concentrated Hb in a phospholipid vesicle. In order to suppress metHb formation for the long term maintenance of oxygen transporting capability in vivo, thiols (cysteine, Cys; homocysteine, Hcy) were studied as reductants of metHb. Hcy showed a suppressive effect on metHb formation, while Cys adversely accelerates metHb formation at the rate of twice the Hb solution without any reductants. The suppression of Cys-induced metHb formation by the addition of superoxide dismutase (SOD) and catalase indicated that Cys was easily oxidized by oxygen and simultaneously generated a large amount of active oxygens. The rate of metHb formation was influenced by PO2 and pH. Furthermore, the reducing systems (methylene blue (MB), NADH or ascorbic acid) were added to the outer aqueous phase of HbV, and the artificial reduction systems constructed through the bilayer membrane were evaluated.
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U2 - 10.3109/10731199709118895
DO - 10.3109/10731199709118895
M3 - Article
C2 - 9083624
AN - SCOPUS:0030945980
SN - 1073-1199
VL - 25
SP - 31
EP - 41
JO - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
JF - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
IS - 1-2
ER -