Construction of manganese porphyrin modified hemoglobin complex and its antioxidant activities

Hidenori Murata; Yuji Ito; Tomoyuki Shimpo; Kenichi Oyaizu; Makoto Yuasa

doi:10.1295/koron.65.277

Construction of manganese porphyrin modified hemoglobin complex and its antioxidant activities

Hidenori Murata^*, Yuji Ito, Tomoyuki Shimpo, Kenichi Oyaizu, Makoto Yuasa

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Manganese 5,10,15,20-tetrakis(N-methyl-2-yl) porphyrin (MnT2MePyP) was introduced to hemoglobin to use it as a drug carrier. Reconstituted hemoglobin was reacted with pyridoxal-5-phosphate and borohydride to yield pyridoxalated reconstituted hemoglobin (PLPrHb). The reaction of PLPrHb with activated polyethyleneglycol gave pegylated reconstituted hemoglobin (PEGrHb). PEGrHb was polymerized using glutaraldehyde to yield cross-linked pegylated reconstituted hemoglobin (Cross-link PEGrHb). The number average moleculer weight of Cross-link PEGrHb was estimated to be 1.0 _Xt 10⁵ by GPC. The modification of hemoglobin was checked by using SDS-PAGE. Stopped flow analysis showed a high SOD activity of Cross-link PEGrHb. A pharmacokinetic study of ¹²⁵I-labelled Cross-link PEGrHb that was infused into mice revealed a long-term blood circulation. The novel Manganese porphyrin modified hemoglobin complex was found to remain long-term in the blood circulation without being removed by excretion. These results showed that this complex can be used as a high performance antioxidant.

Original language	English
Pages (from-to)	277-282
Number of pages	6
Journal	KOBUNSHI RONBUNSHU
Volume	65
Issue number	4
DOIs	https://doi.org/10.1295/koron.65.277
Publication status	Published - 2008 Apr
Externally published	Yes

Keywords

Antioxidant
Cross-link
Hemoglobin
Manganese porphyrin

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Materials Science (miscellaneous)
Environmental Science(all)
Polymers and Plastics

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10.1295/koron.65.277

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title = "Construction of manganese porphyrin modified hemoglobin complex and its antioxidant activities",

abstract = "Manganese 5,10,15,20-tetrakis(N-methyl-2-yl) porphyrin (MnT2MePyP) was introduced to hemoglobin to use it as a drug carrier. Reconstituted hemoglobin was reacted with pyridoxal-5-phosphate and borohydride to yield pyridoxalated reconstituted hemoglobin (PLPrHb). The reaction of PLPrHb with activated polyethyleneglycol gave pegylated reconstituted hemoglobin (PEGrHb). PEGrHb was polymerized using glutaraldehyde to yield cross-linked pegylated reconstituted hemoglobin (Cross-link PEGrHb). The number average moleculer weight of Cross-link PEGrHb was estimated to be 1.0 _Xt 105 by GPC. The modification of hemoglobin was checked by using SDS-PAGE. Stopped flow analysis showed a high SOD activity of Cross-link PEGrHb. A pharmacokinetic study of 125I-labelled Cross-link PEGrHb that was infused into mice revealed a long-term blood circulation. The novel Manganese porphyrin modified hemoglobin complex was found to remain long-term in the blood circulation without being removed by excretion. These results showed that this complex can be used as a high performance antioxidant.",

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author = "Hidenori Murata and Yuji Ito and Tomoyuki Shimpo and Kenichi Oyaizu and Makoto Yuasa",

year = "2008",

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doi = "10.1295/koron.65.277",

language = "English",

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pages = "277--282",

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T1 - Construction of manganese porphyrin modified hemoglobin complex and its antioxidant activities

AU - Murata, Hidenori

AU - Ito, Yuji

AU - Shimpo, Tomoyuki

AU - Oyaizu, Kenichi

AU - Yuasa, Makoto

PY - 2008/4

Y1 - 2008/4

N2 - Manganese 5,10,15,20-tetrakis(N-methyl-2-yl) porphyrin (MnT2MePyP) was introduced to hemoglobin to use it as a drug carrier. Reconstituted hemoglobin was reacted with pyridoxal-5-phosphate and borohydride to yield pyridoxalated reconstituted hemoglobin (PLPrHb). The reaction of PLPrHb with activated polyethyleneglycol gave pegylated reconstituted hemoglobin (PEGrHb). PEGrHb was polymerized using glutaraldehyde to yield cross-linked pegylated reconstituted hemoglobin (Cross-link PEGrHb). The number average moleculer weight of Cross-link PEGrHb was estimated to be 1.0 _Xt 105 by GPC. The modification of hemoglobin was checked by using SDS-PAGE. Stopped flow analysis showed a high SOD activity of Cross-link PEGrHb. A pharmacokinetic study of 125I-labelled Cross-link PEGrHb that was infused into mice revealed a long-term blood circulation. The novel Manganese porphyrin modified hemoglobin complex was found to remain long-term in the blood circulation without being removed by excretion. These results showed that this complex can be used as a high performance antioxidant.

AB - Manganese 5,10,15,20-tetrakis(N-methyl-2-yl) porphyrin (MnT2MePyP) was introduced to hemoglobin to use it as a drug carrier. Reconstituted hemoglobin was reacted with pyridoxal-5-phosphate and borohydride to yield pyridoxalated reconstituted hemoglobin (PLPrHb). The reaction of PLPrHb with activated polyethyleneglycol gave pegylated reconstituted hemoglobin (PEGrHb). PEGrHb was polymerized using glutaraldehyde to yield cross-linked pegylated reconstituted hemoglobin (Cross-link PEGrHb). The number average moleculer weight of Cross-link PEGrHb was estimated to be 1.0 _Xt 105 by GPC. The modification of hemoglobin was checked by using SDS-PAGE. Stopped flow analysis showed a high SOD activity of Cross-link PEGrHb. A pharmacokinetic study of 125I-labelled Cross-link PEGrHb that was infused into mice revealed a long-term blood circulation. The novel Manganese porphyrin modified hemoglobin complex was found to remain long-term in the blood circulation without being removed by excretion. These results showed that this complex can be used as a high performance antioxidant.

KW - Antioxidant

KW - Cross-link

KW - Hemoglobin

KW - Manganese porphyrin

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Construction of manganese porphyrin modified hemoglobin complex and its antioxidant activities

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Keywords

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