Continous hydrogen production by immobilized whole cells of Clostridium butyricum

Isao Karube*, Tadashi Matsunaga, Shinya Tsuru, Shuichi Suzuki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

139 Citations (Scopus)


Clostridium butyricum IFO 3847 was immobilized in polyacrylamide gel. Increase of the polymer concentration decreased hydrogen evolution from glucose by whole cells. The immobilized cells continuously evolved hydrogen from glucose under aerobic conditions. The pH stability of hydrogen production increased with immobilization, and the optimum temperature was 37°C. No difference in the amount of evolved hydrogen was observed after storage for one month at 5°C.

Original languageEnglish
Pages (from-to)338-343
Number of pages6
JournalBBA - General Subjects
Issue number2
Publication statusPublished - 1976 Sept 24
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)


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