Control of cell adhesion and detachment using-temperature and thermoresponsive copolymer grafted culture surfaces

Yukiko Tsuda, Akihiko Kikuchi, Masayuki Yamato, Yasuhisa Sakurai, Mitsuo Umezu, Teruo Okano*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

93 Citations (Scopus)


The hydrophobic monomer, n-butyl methacrylate (BMA) has been incorporated into thermoresponsive poly(N-isopropylacrylamide) (PIPAAm) to lower PIPAAm phase transition temperatures necessary for systematically regulating cell adhesion on and detachment from culture dishes at controlled temperatures. Poly(IPAAm-co-BMA)-grafted dishes were prepared by electron beam irradiation methods, systematically changing BMA content in the feed. Copolymer-grafted surfaces decreased grafted polymer transition temperatures with increasing BMA content as shown by water wettabilities compared to homopolymer PIPAAm-grafted surfaces. Bovine endothelial cells readily adhered and proliferated on copolymer-grafted surfaces above collapse temperature at 37°C, finally reaching confluence. Cell sheet detachment behavior from copolymer-grafted surfaces depended on the culture temperature and BMA content. In conclusion, cell attachment/detachment can be controlled to an arbitrary temperature by varying the content of hydrophobic monomer incorporated into PIPAAm grafted to culture surfaces.

Original languageEnglish
Pages (from-to)70-78
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Issue number1
Publication statusPublished - 2004 Apr 1
Externally publishedYes


  • Cell culture
  • Cell sheet
  • Copolymer grafting
  • N-butyl methacrylate
  • Poly(N-isopropylacrylamide)
  • Temperature responsive surfaces

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys


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