Conversion of phenylpyruvate to l-phenylalanine by immobilized Clostridium butyricum-alanine dehydrogenase-Micrococcus luteus under hydrogen high pressure

Tadashi Matsunaga; M. Higashijima; H. Nakatsugawa; S. Nishimura; T. Kitamura; M. Tsuji; T. Kawaguchi

doi:10.1007/BF00257246

Conversion of phenylpyruvate to l-phenylalanine by immobilized Clostridium butyricum-alanine dehydrogenase-Micrococcus luteus under hydrogen high pressure

Tadashi Matsunaga^*, M. Higashijima, H. Nakatsugawa, S. Nishimura, T. Kitamura, M. Tsuji, T. Kawaguchi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Alanine was the best amino donor among various amino acids and NH₄Cl for the phenylalanine production of Micrococus luteus. l-Alanine was regenerated at the rate of 9.2μ moles/min/g dry cells from NH₄Cl and pyruvate by immobilized Clostridium butyricum-alanine dehydrogenase. l-Phenylalanine was continuously produced from hydrogen, NH₄Cl and phenylpyruvate by coupling immobilized C. butyricum, alanine dehydrogenase and M. luteus. The rate of phenylalanine production was 1.74 μ moles/min/g dry cells.

Original language	English
Pages (from-to)	11-14
Number of pages	4
Journal	Applied Microbiology and Biotechnology
Volume	27
Issue number	1
DOIs	https://doi.org/10.1007/BF00257246
Publication status	Published - 1987 Oct
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

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title = "Conversion of phenylpyruvate to l-phenylalanine by immobilized Clostridium butyricum-alanine dehydrogenase-Micrococcus luteus under hydrogen high pressure",

abstract = "Alanine was the best amino donor among various amino acids and NH4Cl for the phenylalanine production of Micrococus luteus. l-Alanine was regenerated at the rate of 9.2μ moles/min/g dry cells from NH4Cl and pyruvate by immobilized Clostridium butyricum-alanine dehydrogenase. l-Phenylalanine was continuously produced from hydrogen, NH4Cl and phenylpyruvate by coupling immobilized C. butyricum, alanine dehydrogenase and M. luteus. The rate of phenylalanine production was 1.74 μ moles/min/g dry cells.",

author = "Tadashi Matsunaga and M. Higashijima and H. Nakatsugawa and S. Nishimura and T. Kitamura and M. Tsuji and T. Kawaguchi",

year = "1987",

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doi = "10.1007/BF00257246",

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T1 - Conversion of phenylpyruvate to l-phenylalanine by immobilized Clostridium butyricum-alanine dehydrogenase-Micrococcus luteus under hydrogen high pressure

AU - Matsunaga, Tadashi

AU - Higashijima, M.

AU - Nakatsugawa, H.

AU - Nishimura, S.

AU - Kitamura, T.

AU - Tsuji, M.

AU - Kawaguchi, T.

PY - 1987/10

Y1 - 1987/10

N2 - Alanine was the best amino donor among various amino acids and NH4Cl for the phenylalanine production of Micrococus luteus. l-Alanine was regenerated at the rate of 9.2μ moles/min/g dry cells from NH4Cl and pyruvate by immobilized Clostridium butyricum-alanine dehydrogenase. l-Phenylalanine was continuously produced from hydrogen, NH4Cl and phenylpyruvate by coupling immobilized C. butyricum, alanine dehydrogenase and M. luteus. The rate of phenylalanine production was 1.74 μ moles/min/g dry cells.

AB - Alanine was the best amino donor among various amino acids and NH4Cl for the phenylalanine production of Micrococus luteus. l-Alanine was regenerated at the rate of 9.2μ moles/min/g dry cells from NH4Cl and pyruvate by immobilized Clostridium butyricum-alanine dehydrogenase. l-Phenylalanine was continuously produced from hydrogen, NH4Cl and phenylpyruvate by coupling immobilized C. butyricum, alanine dehydrogenase and M. luteus. The rate of phenylalanine production was 1.74 μ moles/min/g dry cells.

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ER -

Conversion of phenylpyruvate to l-phenylalanine by immobilized Clostridium butyricum-alanine dehydrogenase-Micrococcus luteus under hydrogen high pressure

Abstract

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