CpG methylation of the CENP-B box reduces human CENP-B binding

Yoshinori Tanaka; Hitoshi Kurumizaka; Shigeyuki Yokoyama

doi:10.1111/j.1432-1033.2004.04406.x

CpG methylation of the CENP-B box reduces human CENP-B binding

Yoshinori Tanaka, Hitoshi Kurumizaka^*, Shigeyuki Yokoyama

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA. Competition analyses revealed that the affinity of CENP-B for the CENP-B box DNA is reduced nearly to the level of nonspecific DNA binding by CpG methylation.

Original language	English
Pages (from-to)	282-289
Number of pages	8
Journal	FEBS Journal
Volume	272
Issue number	1
DOIs	https://doi.org/10.1111/j.1432-1033.2004.04406.x
Publication status	Published - 2005 Jan

Keywords

CENP-B
Centromere
Chromatin
DNA methylation
Heterochromatin

ASJC Scopus subject areas

Biochemistry

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10.1111/j.1432-1033.2004.04406.x

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abstract = "In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA. Competition analyses revealed that the affinity of CENP-B for the CENP-B box DNA is reduced nearly to the level of nonspecific DNA binding by CpG methylation.",

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AU - Tanaka, Yoshinori

AU - Kurumizaka, Hitoshi

AU - Yokoyama, Shigeyuki

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N2 - In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA. Competition analyses revealed that the affinity of CENP-B for the CENP-B box DNA is reduced nearly to the level of nonspecific DNA binding by CpG methylation.

AB - In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA. Competition analyses revealed that the affinity of CENP-B for the CENP-B box DNA is reduced nearly to the level of nonspecific DNA binding by CpG methylation.

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CpG methylation of the CENP-B box reduces human CENP-B binding

Abstract

Keywords

ASJC Scopus subject areas

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