Cross-sectional imaging of absorption distribution in biological tissue using backscattered light

A technique was developed to reconstruct the cross-sectional image of the absorption distribution in a diffuse medium using backscattered light. In this technique, we illuminate an object with an ultra-short pulse, and measure the time-resolved pulse shape of the light backscattered from the object. The absorption distribution of the scattering object can be estimated using the propagation-path distribution of photons at each detection time and the optical impulse response of backscattered light. In a simulation, the effectiveness of this technique was verified in the cases of a layered absorber and a three dimensional absorber. The nonlinear relationship between the depth of the probing region and the propagation time was clarified. The accuracy of the image reconstruction was significantly improved by the aperiodic sampling of the backscattered impulse response according to the nonlinear relation. The feasibility of the proposed technique was verified in the experiment with a model phantom.