Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1

Takeyuki Suzuki; Yoshiko Okamura; Atsushi Arakaki; Haruko Takeyama; Tadashi Matsunaga

doi:10.1016/j.febslet.2007.06.047

Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1

Takeyuki Suzuki, Yoshiko Okamura, Atsushi Arakaki, Haruko Takeyama, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A non-magnetic mutant of Magnetospirillum magneticum AMB-1 (NMA61), harboring a defective gene located in ORF4 (gene ID: amb4111) was generated by transposon mutagenesis. Biochemical characterization of the gene product of ORF4 revealed that it was localized in the cytoplasm and displayed ATPase activity. The ability of NMA61 to take up iron was severely compromised. Ferrous ion concentration in the medium decreased more with the wild-type than with NMA61, while the iron content in the cytoplasmic fraction of NMA61 was much lower than the wild-type strain. This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB-1.

Original language	English
Pages (from-to)	3443-3448
Number of pages	6
Journal	FEBS Letters
Volume	581
Issue number	18
DOIs	https://doi.org/10.1016/j.febslet.2007.06.047
Publication status	Published - 2007 Jul 24
Externally published	Yes

Keywords

ATPase
Cytoplasmic iron concentration
Ferrous ion transport
Magnetospirillum magneticum AMB-1
Magnetotactic bacteria

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/j.febslet.2007.06.047

Cite this

@article{edcd14f557cb4ef2825374b64ad9fe7b,

title = "Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1",

abstract = "A non-magnetic mutant of Magnetospirillum magneticum AMB-1 (NMA61), harboring a defective gene located in ORF4 (gene ID: amb4111) was generated by transposon mutagenesis. Biochemical characterization of the gene product of ORF4 revealed that it was localized in the cytoplasm and displayed ATPase activity. The ability of NMA61 to take up iron was severely compromised. Ferrous ion concentration in the medium decreased more with the wild-type than with NMA61, while the iron content in the cytoplasmic fraction of NMA61 was much lower than the wild-type strain. This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB-1.",

keywords = "ATPase, Cytoplasmic iron concentration, Ferrous ion transport, Magnetospirillum magneticum AMB-1, Magnetotactic bacteria",

author = "Takeyuki Suzuki and Yoshiko Okamura and Atsushi Arakaki and Haruko Takeyama and Tadashi Matsunaga",

note = "Funding Information: This work was funded in part by a Grant-in-Aid for Scientific Research (A), No. 18206084 from the Ministry of Education, Science, Sports, and Culture of Japan. ",

year = "2007",

month = jul,

day = "24",

doi = "10.1016/j.febslet.2007.06.047",

language = "English",

volume = "581",

pages = "3443--3448",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "18",

}

TY - JOUR

T1 - Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1

AU - Suzuki, Takeyuki

AU - Okamura, Yoshiko

AU - Arakaki, Atsushi

AU - Takeyama, Haruko

AU - Matsunaga, Tadashi

N1 - Funding Information: This work was funded in part by a Grant-in-Aid for Scientific Research (A), No. 18206084 from the Ministry of Education, Science, Sports, and Culture of Japan.

PY - 2007/7/24

Y1 - 2007/7/24

N2 - A non-magnetic mutant of Magnetospirillum magneticum AMB-1 (NMA61), harboring a defective gene located in ORF4 (gene ID: amb4111) was generated by transposon mutagenesis. Biochemical characterization of the gene product of ORF4 revealed that it was localized in the cytoplasm and displayed ATPase activity. The ability of NMA61 to take up iron was severely compromised. Ferrous ion concentration in the medium decreased more with the wild-type than with NMA61, while the iron content in the cytoplasmic fraction of NMA61 was much lower than the wild-type strain. This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB-1.

AB - A non-magnetic mutant of Magnetospirillum magneticum AMB-1 (NMA61), harboring a defective gene located in ORF4 (gene ID: amb4111) was generated by transposon mutagenesis. Biochemical characterization of the gene product of ORF4 revealed that it was localized in the cytoplasm and displayed ATPase activity. The ability of NMA61 to take up iron was severely compromised. Ferrous ion concentration in the medium decreased more with the wild-type than with NMA61, while the iron content in the cytoplasmic fraction of NMA61 was much lower than the wild-type strain. This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB-1.

KW - ATPase

KW - Cytoplasmic iron concentration

KW - Ferrous ion transport

KW - Magnetospirillum magneticum AMB-1

KW - Magnetotactic bacteria

UR - http://www.scopus.com/inward/record.url?scp=34447280419&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447280419&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2007.06.047

DO - 10.1016/j.febslet.2007.06.047

M3 - Article

C2 - 17618623

AN - SCOPUS:34447280419

SN - 0014-5793

VL - 581

SP - 3443

EP - 3448

JO - FEBS Letters

JF - FEBS Letters

IS - 18

ER -

Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this