Cytotoxicity of synthetic barium hydroxyapatite

Masanori Kikuchi*, Astsushi Yamazaki, Masaru Akao, Hideki Aoki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Barium hydroxyapatite (Ba10(PO4)6(OH)2, Ba-HAp) was synthesized by a wet method using Ba(OH)2 · 8H2O and (NH4)2HPO4 as starting materials. The Ba-HAp obtained had a Ba/P atomic ratio of 1.76 and contained CO3 groups. The Ba-HAp was sintered at 1073 K for 12 hours. The sintered Ba-HAp had a three point bending strength of 29 MPa and Young's modulus of 27 GPa. Cytotoxicity of the sintered bodies and particles was tested using L-cells. The sintered Ba-HAp showed no cytotoxicity, and the cells were closely in contact with the surfaces of sintered Ba-HAp. Morphological observation of the cell around the Ba-HAp particles also showed no cytotoxicity. However, cell growth was inhibited by Ca adsorption on the Ba-HAp particles. These results suggested that the Ba-HAp had no cytotoxicity and can be applied as a bioactive X-ray opaque material.

Original languageEnglish
Pages (from-to)405-413
Number of pages9
JournalBio-Medical Materials and Engineering
Issue number6
Publication statusPublished - 1996 Dec 1


  • Barium hydroxyapatite
  • Ca adsorption
  • Cell growth inhibition
  • Cytotoxicity
  • Mechanical strength

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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