Daily expression of clock genes in whole blood cells in healthy subjects and a patient with circadian rhythm sleep disorder

Mieko Takimoto, Akinobu Hamada, Akemi Tomoda, Shigehiro Ohdo, Takafumi Ohmura, Hisao Sakato, Junko Kawatani, Takako Jodoi, Hiroo Nakagawa, Hideyuki Terazono, Satoru Koyanagi, Shun Higuchi, Miyuki Kimura, Hiroshi Tukikawa, Shin Irie, Hideyuki Saito*, Teruhisa Miike

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)


In recent years, circadian rhythm sleep disorders in humans have been increasing. Clinical features characteristic of this disorder are well known, but the specific causes remain unknown. However, various derangements of circadian expression of the clock gene are a probable cause of this disease. We have attempted to elucidate the relationship between the expression of the clock genes in whole blood cells and the clinical features characteristic of this disorder. In this study, we indicate the daily expression of clock genes period (Per) 1, 2, 3, Bmal1, and Clock in whole blood cells in 12 healthy male subjects. The peak phase of Per1, Per2, and Per3 appeared in the early morning, whereas that of Bmal1 and Clock appeared in the midnight hours. Furthermore, in one patient case with circadian rhythm sleep disorder, we observed variations of the peak phase in clock genes by treatments such as light therapy, exercise therapy, and medicinal therapy. This study suggested that the monitoring of human clock genes in whole blood cells, which may be functionally important for the molecular control of the circadian pacemaker as well as in suprachiasmatic nucleus, might be useful to evaluate internal synchronization.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Issue number5 58-5
Publication statusPublished - 2005 Nov
Externally publishedYes


  • Circadian pacemaker
  • Internal synchronization

ASJC Scopus subject areas

  • Physiology


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