TY - JOUR
T1 - Decrease of citric acid produced by Aspergillus Niger through disruption of the gene encoding a putative mitochondrial citrate-oxoglutarate shuttle protein
AU - Kirimura, Kohtaro
AU - Kobayashi, Keiichi
AU - Yoshioka, Isato
N1 - Funding Information:
We thank Mr. Shota Ijichi and Ms. Yuka Ueda for technical assistance, and Dr. Masanori Suzuki for helpful discussion of this work. This paper is a part of the outcome of research performed under a Waseda University Grant for Special Research Projects [2018A-031].
PY - 2019
Y1 - 2019
N2 - The transporter that exports citric acid (CA) generated in mitochondria to the cytosol is an important component of the CA production machinery of Aspergillus Niger. In this report, we cloned and identified the gene cocA, encoding a 33.7-kDa putative mitochondrial citrate-oxoglutarate shuttle protein of the CA hyper-producer A. Niger WU-2223L. The amount of CA produced by a representative cocA disruptant (35 g/L) was significantly lower than that produced by strain WU-2223L (63 g/L) after culture for 12 days under CA production conditions, and the phenotype of the cocA disruptant differed in part from that of strain WU-2223L. A cocA disruptant complemented with cocA exhibited the same phenotypes as those of strain WU-2223L. This report is the first to show that cocA and its protein product clearly contribute to substantial CA production by A. Niger, and provides a significant insight into microbial organic acid production by fermentation.
AB - The transporter that exports citric acid (CA) generated in mitochondria to the cytosol is an important component of the CA production machinery of Aspergillus Niger. In this report, we cloned and identified the gene cocA, encoding a 33.7-kDa putative mitochondrial citrate-oxoglutarate shuttle protein of the CA hyper-producer A. Niger WU-2223L. The amount of CA produced by a representative cocA disruptant (35 g/L) was significantly lower than that produced by strain WU-2223L (63 g/L) after culture for 12 days under CA production conditions, and the phenotype of the cocA disruptant differed in part from that of strain WU-2223L. A cocA disruptant complemented with cocA exhibited the same phenotypes as those of strain WU-2223L. This report is the first to show that cocA and its protein product clearly contribute to substantial CA production by A. Niger, and provides a significant insight into microbial organic acid production by fermentation.
KW - Aspergillus Niger
KW - Citrate transporter
KW - Citrate-oxoglutarate shuttle protein
KW - Citric acid production
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U2 - 10.1080/09168451.2019.1574205
DO - 10.1080/09168451.2019.1574205
M3 - Article
C2 - 30720390
AN - SCOPUS:85068590637
SN - 0916-8451
VL - 83
SP - 1538
EP - 1546
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 8
ER -