TY - JOUR
T1 - Detection of epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) using a fully automated system with a nano-scale engineered biomagnetite
AU - Maruyama, Kohei
AU - Takeyama, Haruko
AU - Mori, Tetsushi
AU - Ohshima, Keiichi
AU - Ogura, Shun Ichiro
AU - Mochizuki, Toru
AU - Matsunaga, Tadashi
N1 - Funding Information:
We would like to thank Mr. Yoda, Mr. Suzuki, Mr. Nagai, Mr. Nemoto, Mr. Sugiyama, Mr. Tsukada and Mr. Kato of JUKI Corporation for prototyping the fully automated system. This work was funded in part by NEDO's Subsidy for FY2002 University-initiated Practical Business Creation R&D Program and Scientific Research program from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2007/4/15
Y1 - 2007/4/15
N2 - A fully automated system using nano-scale engineered biomagnetite was developed to detect mutations in the epidermal growth factor receptor (EGFR) gene in non-small cell lung cancer (NSCLC). Bacterial magnetic particles (BacMPs) were isolated from the magnetic bacterium Magnetospirillum magneticum AMB-1 and conjugated to streptavidin. Biotin-labeled target PCR products were then captured with the BacMPs, hybridized with the detection probe and detected by fluorescence signaling. The process was performed using a newly designed automated processor equipped with an XYZ mobile arm containing a 96-way automated pipetter, reagent dispenser and fluorescence detector. Two types of somatic mutations (in-frame deletions and point substitutions) in the EGFR gene were successfully identified within 3.5 h using this system, suggesting that this system could be used in clinical tests of EGFR gene mutations in lung cancer, and potentially other cancer, patients. Additionally, a very low mutation rate could be detected in these samples.
AB - A fully automated system using nano-scale engineered biomagnetite was developed to detect mutations in the epidermal growth factor receptor (EGFR) gene in non-small cell lung cancer (NSCLC). Bacterial magnetic particles (BacMPs) were isolated from the magnetic bacterium Magnetospirillum magneticum AMB-1 and conjugated to streptavidin. Biotin-labeled target PCR products were then captured with the BacMPs, hybridized with the detection probe and detected by fluorescence signaling. The process was performed using a newly designed automated processor equipped with an XYZ mobile arm containing a 96-way automated pipetter, reagent dispenser and fluorescence detector. Two types of somatic mutations (in-frame deletions and point substitutions) in the EGFR gene were successfully identified within 3.5 h using this system, suggesting that this system could be used in clinical tests of EGFR gene mutations in lung cancer, and potentially other cancer, patients. Additionally, a very low mutation rate could be detected in these samples.
KW - Allele-specific oligonucleotide hybridization
KW - Automated system
KW - Bacterial magnetic particles (BacMPs)
KW - Epidermal growth factor receptor (EGFR) mutation
KW - Nano-scale engineered biomagnetite
KW - Non-small cell lung cancer (NSCLC)
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U2 - 10.1016/j.bios.2006.11.018
DO - 10.1016/j.bios.2006.11.018
M3 - Article
C2 - 17187977
AN - SCOPUS:33847627885
SN - 0956-5663
VL - 22
SP - 2282
EP - 2288
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 9-10
ER -
