Determination of cholesterol with a laboratory-built chemiluminescence system

Akiyoshi Taniguchi; Yuzuru Hayashi; Hidetaka Yuki

doi:10.1016/S0003-2670(00)86033-X

Determination of cholesterol with a laboratory-built chemiluminescence system

Akiyoshi Taniguchi^*, Yuzuru Hayashi, Hidetaka Yuki

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Chemiluminescence measurement by a laboratory-built computer system equipped with an automated injection device is applied to a cholesterol enzymatic assay. The hydrogen peroxide produced by cholesterol esterase and oxidase is mixed rapidly with horseradish peroxidase-luminol solution (pH 10.5) and the light emission data are processed by a Fourier-transform digital filter to produce peak-height measurements. A blood serum volume of 0.2 μl and an observation time of 0.5 s are required.

Original language	English
Pages (from-to)	95-100
Number of pages	6
Journal	Analytica Chimica Acta
Volume	188
Issue number	C
DOIs	https://doi.org/10.1016/S0003-2670(00)86033-X
Publication status	Published - 1986
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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10.1016/S0003-2670(00)86033-X

Cite this

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title = "Determination of cholesterol with a laboratory-built chemiluminescence system",

abstract = "Chemiluminescence measurement by a laboratory-built computer system equipped with an automated injection device is applied to a cholesterol enzymatic assay. The hydrogen peroxide produced by cholesterol esterase and oxidase is mixed rapidly with horseradish peroxidase-luminol solution (pH 10.5) and the light emission data are processed by a Fourier-transform digital filter to produce peak-height measurements. A blood serum volume of 0.2 μl and an observation time of 0.5 s are required.",

author = "Akiyoshi Taniguchi and Yuzuru Hayashi and Hidetaka Yuki",

year = "1986",

doi = "10.1016/S0003-2670(00)86033-X",

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T1 - Determination of cholesterol with a laboratory-built chemiluminescence system

AU - Taniguchi, Akiyoshi

AU - Hayashi, Yuzuru

AU - Yuki, Hidetaka

PY - 1986

Y1 - 1986

N2 - Chemiluminescence measurement by a laboratory-built computer system equipped with an automated injection device is applied to a cholesterol enzymatic assay. The hydrogen peroxide produced by cholesterol esterase and oxidase is mixed rapidly with horseradish peroxidase-luminol solution (pH 10.5) and the light emission data are processed by a Fourier-transform digital filter to produce peak-height measurements. A blood serum volume of 0.2 μl and an observation time of 0.5 s are required.

AB - Chemiluminescence measurement by a laboratory-built computer system equipped with an automated injection device is applied to a cholesterol enzymatic assay. The hydrogen peroxide produced by cholesterol esterase and oxidase is mixed rapidly with horseradish peroxidase-luminol solution (pH 10.5) and the light emission data are processed by a Fourier-transform digital filter to produce peak-height measurements. A blood serum volume of 0.2 μl and an observation time of 0.5 s are required.

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U2 - 10.1016/S0003-2670(00)86033-X

DO - 10.1016/S0003-2670(00)86033-X

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AN - SCOPUS:0002333727

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JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - C

ER -

Determination of cholesterol with a laboratory-built chemiluminescence system

Abstract

ASJC Scopus subject areas

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