Determination of trace levels of fatty acid metal salts by high-performance liquid chromatography with fluorescence prelabeling

Katsumi Hayashi; Jiro Kawase; Koichi Yoshimura; Katsutoshi Ara; Kazuro Tsuji

doi:10.1016/0003-2697(84)90223-9

Determination of trace levels of fatty acid metal salts by high-performance liquid chromatography with fluorescence prelabeling

Katsumi Hayashi^*, Jiro Kawase, Koichi Yoshimura, Katsutoshi Ara, Kazuro Tsuji

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A simple method is described for picomole determinations of fatty acid metal salts. Fatty acid salts are directly labeled with 4-bromomethyl-7-methoxycoumarin in the presence of excess ethylenediaminetetraacetic acid tripotassium salt without any solvent extractions. The fluorescence derivatives of fatty acids are separated by reverse-phase high-performance liquid chromatography followed by fluorometric detection. The response of each fatty acid (C₈-C₁₈) calcium salt is linear from 1 to 50 μg/ml of samples. The detection limit is about 7 pmol. Good recoveries are obtained for the calcium salts of myrystic acid and soap (C₈-C₁₈, C_18:1,2). The new method is successfully applied to the study on biodegradation of fatty acids in river water.

Original language	English
Pages (from-to)	314-320
Number of pages	7
Journal	Analytical Biochemistry
Volume	136
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(84)90223-9
Publication status	Published - 1984 Feb 1
Externally published	Yes

Keywords

HPLC
fatty acid
fluorescence detection
metal salt
microdetermination
prederivatization

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(84)90223-9

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title = "Determination of trace levels of fatty acid metal salts by high-performance liquid chromatography with fluorescence prelabeling",

abstract = "A simple method is described for picomole determinations of fatty acid metal salts. Fatty acid salts are directly labeled with 4-bromomethyl-7-methoxycoumarin in the presence of excess ethylenediaminetetraacetic acid tripotassium salt without any solvent extractions. The fluorescence derivatives of fatty acids are separated by reverse-phase high-performance liquid chromatography followed by fluorometric detection. The response of each fatty acid (C8-C18) calcium salt is linear from 1 to 50 μg/ml of samples. The detection limit is about 7 pmol. Good recoveries are obtained for the calcium salts of myrystic acid and soap (C8-C18, C18:1,2). The new method is successfully applied to the study on biodegradation of fatty acids in river water.",

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author = "Katsumi Hayashi and Jiro Kawase and Koichi Yoshimura and Katsutoshi Ara and Kazuro Tsuji",

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T1 - Determination of trace levels of fatty acid metal salts by high-performance liquid chromatography with fluorescence prelabeling

AU - Hayashi, Katsumi

AU - Kawase, Jiro

AU - Yoshimura, Koichi

AU - Ara, Katsutoshi

AU - Tsuji, Kazuro

PY - 1984/2/1

Y1 - 1984/2/1

N2 - A simple method is described for picomole determinations of fatty acid metal salts. Fatty acid salts are directly labeled with 4-bromomethyl-7-methoxycoumarin in the presence of excess ethylenediaminetetraacetic acid tripotassium salt without any solvent extractions. The fluorescence derivatives of fatty acids are separated by reverse-phase high-performance liquid chromatography followed by fluorometric detection. The response of each fatty acid (C8-C18) calcium salt is linear from 1 to 50 μg/ml of samples. The detection limit is about 7 pmol. Good recoveries are obtained for the calcium salts of myrystic acid and soap (C8-C18, C18:1,2). The new method is successfully applied to the study on biodegradation of fatty acids in river water.

AB - A simple method is described for picomole determinations of fatty acid metal salts. Fatty acid salts are directly labeled with 4-bromomethyl-7-methoxycoumarin in the presence of excess ethylenediaminetetraacetic acid tripotassium salt without any solvent extractions. The fluorescence derivatives of fatty acids are separated by reverse-phase high-performance liquid chromatography followed by fluorometric detection. The response of each fatty acid (C8-C18) calcium salt is linear from 1 to 50 μg/ml of samples. The detection limit is about 7 pmol. Good recoveries are obtained for the calcium salts of myrystic acid and soap (C8-C18, C18:1,2). The new method is successfully applied to the study on biodegradation of fatty acids in river water.

KW - HPLC

KW - fatty acid

KW - fluorescence detection

KW - metal salt

KW - microdetermination

KW - prederivatization

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SN - 0003-2697

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JO - Analytical Biochemistry

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ER -

Determination of trace levels of fatty acid metal salts by high-performance liquid chromatography with fluorescence prelabeling

Abstract

Keywords

ASJC Scopus subject areas

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