Development of a bench-top extra-cleanroom for DNA amplification

Hirokazu Takahashi*, Takahiro Satoh, Hiroko Kanahara, Yuji Kubota, Tamaki Hirose, Hiroyuki Yamazaki, Kimiko Yamamoto, Yoshiko Okamura, Taketo Suzuki, Toshiro Kobori

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Prevention of airborne contamination has become an important factor in biotechnology; however, conventional laminar-airflow cabinets (LAF-cabinets) are no longer sufficient as a countermeasure against nano-sized airborne contaminants in the laboratory. Here we present a bench-top extra-cleanroom classified as ISO-1 that can prevent contamination from airborne nanoparticles. This bench-top extra-cleanroom consists of a novel clean-zone-creating system that is equipped with nanofibrous, nonwoven filters. In addition, the cleanroom is also equipped with an ionizer to prevent plasticware from collecting dust by electrostatic charge attraction. This combination of features allows the cleanroom to prevent DNA contamination derived from airborne nanoparticles. Our extra-cleanroom with ionizer could be useful in various areas of biotechnology that are easily affected by airborne contaminants.

Original languageEnglish
Pages (from-to)42-46
Number of pages5
JournalBioTechniques
Volume61
Issue number1
DOIs
Publication statusPublished - 2016 Jul
Externally publishedYes

Keywords

  • Airborne nanoparticle contamination
  • Bench-top extra-cleanroom
  • Random RNA primers
  • Whole-genome amplification
  • ø29 DNA polymerase

ASJC Scopus subject areas

  • Biotechnology
  • General Biochemistry,Genetics and Molecular Biology

Fingerprint

Dive into the research topics of 'Development of a bench-top extra-cleanroom for DNA amplification'. Together they form a unique fingerprint.

Cite this