Development of a microdevice for cellular analysis integrated with microvalves

We have developed a microdevice for cellular analysis, which has a microwell for cell culture and on-chip microvalves for sample injection. In order to reduce dead volume in the sample injector, the device structure is designed to minimize distance between the microwell and the sample injector. Two types of PDMS are used for the lid of the microdevice and the diaphragm of the microvalves, which have an advantage of high transparency and that of bio-compatibility, respectively. Mechanical property and gas permeability of the two types of PDMS are examined and the results show that the both PDMS are applicable to the microdevice. Using the fabricated prototype device, sample injection of the order of nano-liter is demonstrated successfully.