Development of a rapid isolation procedure for coccolith ultrafine particles produced by coccolithophorid algae

Hiroyuki Takano; Eichi Manabe; Morio Hirano; Megumi Okazaki; J. Grant Burgess; Noriyuki Nakamura; Tadashi Matsunaga

doi:10.1007/BF02918993

Development of a rapid isolation procedure for coccolith ultrafine particles produced by coccolithophorid algae

Hiroyuki Takano, Eichi Manabe, Morio Hirano, Megumi Okazaki, J. Grant Burgess, Noriyuki Nakamura, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A rapid procedure for effective purification of large quantities of coccolith ultrafine particles from marine algae is reported. Coccoliths are detached from cells by optimized sonication in the presence of 50 m M NaHCO₃. Contaminating cell debris is then removed from coccoliths by cycles of washing and floatation. Coccolith particles were purified from Emiliania huxleyi and Pleurochrysis carterae. The surface area of these particles is three to five times greater than synthetic calcite particles. Glucose oxidase and uricase have been immobilized onto purified coccolith ultrafine particles to illustrate their potential as a support material for biotechnological application.

Original language	English
Pages (from-to)	239-247
Number of pages	9
Journal	Applied Biochemistry and Biotechnology
Volume	39-40
Issue number	1
DOIs	https://doi.org/10.1007/BF02918993
Publication status	Published - 1993 Sept
Externally published	Yes

Keywords

calcite ultrafine particles
coccolith
Coccolithophorid algae
enzyme immobilization

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF02918993

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abstract = "A rapid procedure for effective purification of large quantities of coccolith ultrafine particles from marine algae is reported. Coccoliths are detached from cells by optimized sonication in the presence of 50 m M NaHCO3. Contaminating cell debris is then removed from coccoliths by cycles of washing and floatation. Coccolith particles were purified from Emiliania huxleyi and Pleurochrysis carterae. The surface area of these particles is three to five times greater than synthetic calcite particles. Glucose oxidase and uricase have been immobilized onto purified coccolith ultrafine particles to illustrate their potential as a support material for biotechnological application.",

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author = "Hiroyuki Takano and Eichi Manabe and Morio Hirano and Megumi Okazaki and Burgess, {J. Grant} and Noriyuki Nakamura and Tadashi Matsunaga",

year = "1993",

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AU - Takano, Hiroyuki

AU - Manabe, Eichi

AU - Hirano, Morio

AU - Okazaki, Megumi

AU - Burgess, J. Grant

AU - Nakamura, Noriyuki

AU - Matsunaga, Tadashi

PY - 1993/9

Y1 - 1993/9

N2 - A rapid procedure for effective purification of large quantities of coccolith ultrafine particles from marine algae is reported. Coccoliths are detached from cells by optimized sonication in the presence of 50 m M NaHCO3. Contaminating cell debris is then removed from coccoliths by cycles of washing and floatation. Coccolith particles were purified from Emiliania huxleyi and Pleurochrysis carterae. The surface area of these particles is three to five times greater than synthetic calcite particles. Glucose oxidase and uricase have been immobilized onto purified coccolith ultrafine particles to illustrate their potential as a support material for biotechnological application.

AB - A rapid procedure for effective purification of large quantities of coccolith ultrafine particles from marine algae is reported. Coccoliths are detached from cells by optimized sonication in the presence of 50 m M NaHCO3. Contaminating cell debris is then removed from coccoliths by cycles of washing and floatation. Coccolith particles were purified from Emiliania huxleyi and Pleurochrysis carterae. The surface area of these particles is three to five times greater than synthetic calcite particles. Glucose oxidase and uricase have been immobilized onto purified coccolith ultrafine particles to illustrate their potential as a support material for biotechnological application.

KW - calcite ultrafine particles

KW - coccolith

KW - Coccolithophorid algae

KW - enzyme immobilization

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Development of a rapid isolation procedure for coccolith ultrafine particles produced by coccolithophorid algae

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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