Development of rapid detection system for allergic reaction using rat basophilic leukemia (RBL-1) cells

Noriyuki Nakamura; Shizuko Kumazawa; Koji Sode; Tadashi Matsunaga

doi:10.1016/0925-4005(93)85389-R

Development of rapid detection system for allergic reaction using rat basophilic leukemia (RBL-1) cells

Noriyuki Nakamura^*, Shizuko Kumazawa, Koji Sode, Tadashi Matsunaga

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We have investigated the electrochemical detection system of allergic reaction using rat basophilic leukemia (RBL-1) cells by applying cyclic voltammetry. When the potential of the electrode is run in the range 0-1.0 V versus a saturated calomel electrode (SCE), an anodic peak current appears around 0.34 V. This peak current or RBL-1 cells is attributed to intracellular serotonin. After the addition of allergen, this peak current increases with time and the maximum current (0.82 μA per 2×10⁵ cells) is obtained after 20 min. On the other hand, the current is constant when the allergen is not added. This current increase is attributed to localization of the intracellular serotonin and is specific for allergen.

Original language	English
Pages (from-to)	312-314
Number of pages	3
Journal	Sensors and Actuators: B. Chemical
Volume	13
Issue number	1-3
DOIs	https://doi.org/10.1016/0925-4005(93)85389-R
Publication status	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry
Electrical and Electronic Engineering

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title = "Development of rapid detection system for allergic reaction using rat basophilic leukemia (RBL-1) cells",

abstract = "We have investigated the electrochemical detection system of allergic reaction using rat basophilic leukemia (RBL-1) cells by applying cyclic voltammetry. When the potential of the electrode is run in the range 0-1.0 V versus a saturated calomel electrode (SCE), an anodic peak current appears around 0.34 V. This peak current or RBL-1 cells is attributed to intracellular serotonin. After the addition of allergen, this peak current increases with time and the maximum current (0.82 μA per 2×105 cells) is obtained after 20 min. On the other hand, the current is constant when the allergen is not added. This current increase is attributed to localization of the intracellular serotonin and is specific for allergen.",

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T1 - Development of rapid detection system for allergic reaction using rat basophilic leukemia (RBL-1) cells

AU - Nakamura, Noriyuki

AU - Kumazawa, Shizuko

AU - Sode, Koji

AU - Matsunaga, Tadashi

PY - 1993

Y1 - 1993

N2 - We have investigated the electrochemical detection system of allergic reaction using rat basophilic leukemia (RBL-1) cells by applying cyclic voltammetry. When the potential of the electrode is run in the range 0-1.0 V versus a saturated calomel electrode (SCE), an anodic peak current appears around 0.34 V. This peak current or RBL-1 cells is attributed to intracellular serotonin. After the addition of allergen, this peak current increases with time and the maximum current (0.82 μA per 2×105 cells) is obtained after 20 min. On the other hand, the current is constant when the allergen is not added. This current increase is attributed to localization of the intracellular serotonin and is specific for allergen.

AB - We have investigated the electrochemical detection system of allergic reaction using rat basophilic leukemia (RBL-1) cells by applying cyclic voltammetry. When the potential of the electrode is run in the range 0-1.0 V versus a saturated calomel electrode (SCE), an anodic peak current appears around 0.34 V. This peak current or RBL-1 cells is attributed to intracellular serotonin. After the addition of allergen, this peak current increases with time and the maximum current (0.82 μA per 2×105 cells) is obtained after 20 min. On the other hand, the current is constant when the allergen is not added. This current increase is attributed to localization of the intracellular serotonin and is specific for allergen.

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Development of rapid detection system for allergic reaction using rat basophilic leukemia (RBL-1) cells

Abstract

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