TY - GEN
T1 - Development of size-selective microfluidic platform
AU - Chen, Zheyuan
AU - Yamaguchi, Hirohito
AU - Kameoka, Jun
N1 - Funding Information:
1322 10.1109/EMBC.2019.8857536 0b0000648b6918dd Active orig-research F T F F X F T F Publish IEEE Exosomes are nanosized extracellular vesicles that play a significant role in cell-cell communication. Recently, there is significant interest in exosome-related fundamental research, especially subgroups of exosomes as potential biomarkers for cancer diagnosis and prognosis. In this paper, we report a new size selective isolation method via elastic lift force and nanomembrane filtration and demonstrated the liposome recovery rate of 92.5% from a mixture solution of 1 μm polystyrene beads, 100 nm liposomes and proteins as a proof of concept for exosome isolation. This single microfluidic platform offers an improved approach with short processing time (< 2 hours) and low cost, and shows potential broad applicability to cancer biomarker studies. 1 Chen, Zheyuan Zheyuan Chen Zheyuan Chen Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX, 77840, USA Author 2 Yamaguchi, Hirohito Hirohito Yamaguchi Hirohito Yamaguchi Cancer Research Center, Qatar Biomedical Research Institute, College of Health and Life Sciences, Hamad Bin Khalifa University, Qatar Foundation, Doha, Qatar Author 3 Kameoka, Jun Jun Kameoka Jun Kameoka Department of Electrical and Computer Engineering and the Department of Material Science and Engineering, Texas A&M University, College Station, TX, 77840, USA Author 2019 July 2019 10 2 906297 08857536.pdf 5661-5664 8857536 Microchannels Proteins Extracellular Microfluidics Fluorescence Cancer Force
Publisher Copyright:
© 2019 IEEE.
PY - 2019/7
Y1 - 2019/7
N2 - Exosomes are nanosized extracellular vesicles that play a significant role in cell-cell communication. Recently, there is significant interest in exosome-related fundamental research, especially subgroups of exosomes as potential biomarkers for cancer diagnosis and prognosis. In this paper, we report a new size selective isolation method via elastic lift force and nanomembrane filtration and demonstrated the liposome recovery rate of 92.5% from a mixture solution of 1 μm polystyrene beads, 100 nm liposomes and proteins as a proof of concept for exosome isolation. This single microfluidic platform offers an improved approach with short processing time (< 2 hours) and low cost, and shows potential broad applicability to cancer biomarker studies.
AB - Exosomes are nanosized extracellular vesicles that play a significant role in cell-cell communication. Recently, there is significant interest in exosome-related fundamental research, especially subgroups of exosomes as potential biomarkers for cancer diagnosis and prognosis. In this paper, we report a new size selective isolation method via elastic lift force and nanomembrane filtration and demonstrated the liposome recovery rate of 92.5% from a mixture solution of 1 μm polystyrene beads, 100 nm liposomes and proteins as a proof of concept for exosome isolation. This single microfluidic platform offers an improved approach with short processing time (< 2 hours) and low cost, and shows potential broad applicability to cancer biomarker studies.
UR - http://www.scopus.com/inward/record.url?scp=85077869958&partnerID=8YFLogxK
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U2 - 10.1109/EMBC.2019.8857536
DO - 10.1109/EMBC.2019.8857536
M3 - Conference contribution
C2 - 31947137
AN - SCOPUS:85077869958
T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
SP - 5661
EP - 5664
BT - 2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
Y2 - 23 July 2019 through 27 July 2019
ER -
