Development of the automated circulating tumor cell recovery system with microcavity array

Ryo Negishi; Masahito Hosokawa; Seita Nakamura; Hisashige Kanbara; Masafumi Kanetomo; Yoshihito Kikuhara; Tsuyoshi Tanaka; Tadashi Matsunaga; Tomoko Yoshino

doi:10.1016/j.bios.2014.09.002

Development of the automated circulating tumor cell recovery system with microcavity array

Ryo Negishi, Masahito Hosokawa, Seita Nakamura, Hisashige Kanbara, Masafumi Kanetomo, Yoshihito Kikuhara, Tsuyoshi Tanaka, Tadashi Matsunaga, Tomoko Yoshino^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Circulating tumor cells (CTCs) are well recognized as useful biomarker for cancer diagnosis and potential target of drug discovery for metastatic cancer. Efficient and precise recovery of extremely low concentrations of CTCs from blood has been required to increase the detection sensitivity. Here, an automated system equipped with a microcavity array (MCA) was demonstrated for highly efficient and reproducible CTC recovery. The use of MCA allows selective recovery of cancer cells from whole blood on the basis of differences in size between tumor and blood cells. Intra- and inter-assays revealed that the automated system achieved high efficiency and reproducibility equal to the assay manually performed by well-trained operator. Under optimized assay workflow, the automated system allows efficient and precise cell recovery for non-small cell lung cancer cells spiked in whole blood. The automated CTC recovery system will contribute to high-throughput analysis in the further clinical studies on large cohort of cancer patients.

Original language	English
Pages (from-to)	438-442
Number of pages	5
Journal	Biosensors and Bioelectronics
Volume	67
DOIs	https://doi.org/10.1016/j.bios.2014.09.002
Publication status	Published - 2015 May 5
Externally published	Yes

Keywords

Automated system
Circulating tumor cells
Lung cancer
Whole blood

ASJC Scopus subject areas

Biophysics
Biotechnology
Biomedical Engineering
Electrochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bios.2014.09.002

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title = "Development of the automated circulating tumor cell recovery system with microcavity array",

abstract = "Circulating tumor cells (CTCs) are well recognized as useful biomarker for cancer diagnosis and potential target of drug discovery for metastatic cancer. Efficient and precise recovery of extremely low concentrations of CTCs from blood has been required to increase the detection sensitivity. Here, an automated system equipped with a microcavity array (MCA) was demonstrated for highly efficient and reproducible CTC recovery. The use of MCA allows selective recovery of cancer cells from whole blood on the basis of differences in size between tumor and blood cells. Intra- and inter-assays revealed that the automated system achieved high efficiency and reproducibility equal to the assay manually performed by well-trained operator. Under optimized assay workflow, the automated system allows efficient and precise cell recovery for non-small cell lung cancer cells spiked in whole blood. The automated CTC recovery system will contribute to high-throughput analysis in the further clinical studies on large cohort of cancer patients.",

keywords = "Automated system, Circulating tumor cells, Lung cancer, Whole blood",

author = "Ryo Negishi and Masahito Hosokawa and Seita Nakamura and Hisashige Kanbara and Masafumi Kanetomo and Yoshihito Kikuhara and Tsuyoshi Tanaka and Tadashi Matsunaga and Tomoko Yoshino",

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year = "2015",

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doi = "10.1016/j.bios.2014.09.002",

language = "English",

volume = "67",

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AU - Negishi, Ryo

AU - Hosokawa, Masahito

AU - Nakamura, Seita

AU - Kanbara, Hisashige

AU - Kanetomo, Masafumi

AU - Kikuhara, Yoshihito

AU - Tanaka, Tsuyoshi

AU - Matsunaga, Tadashi

AU - Yoshino, Tomoko

PY - 2015/5/5

Y1 - 2015/5/5

N2 - Circulating tumor cells (CTCs) are well recognized as useful biomarker for cancer diagnosis and potential target of drug discovery for metastatic cancer. Efficient and precise recovery of extremely low concentrations of CTCs from blood has been required to increase the detection sensitivity. Here, an automated system equipped with a microcavity array (MCA) was demonstrated for highly efficient and reproducible CTC recovery. The use of MCA allows selective recovery of cancer cells from whole blood on the basis of differences in size between tumor and blood cells. Intra- and inter-assays revealed that the automated system achieved high efficiency and reproducibility equal to the assay manually performed by well-trained operator. Under optimized assay workflow, the automated system allows efficient and precise cell recovery for non-small cell lung cancer cells spiked in whole blood. The automated CTC recovery system will contribute to high-throughput analysis in the further clinical studies on large cohort of cancer patients.

AB - Circulating tumor cells (CTCs) are well recognized as useful biomarker for cancer diagnosis and potential target of drug discovery for metastatic cancer. Efficient and precise recovery of extremely low concentrations of CTCs from blood has been required to increase the detection sensitivity. Here, an automated system equipped with a microcavity array (MCA) was demonstrated for highly efficient and reproducible CTC recovery. The use of MCA allows selective recovery of cancer cells from whole blood on the basis of differences in size between tumor and blood cells. Intra- and inter-assays revealed that the automated system achieved high efficiency and reproducibility equal to the assay manually performed by well-trained operator. Under optimized assay workflow, the automated system allows efficient and precise cell recovery for non-small cell lung cancer cells spiked in whole blood. The automated CTC recovery system will contribute to high-throughput analysis in the further clinical studies on large cohort of cancer patients.

KW - Automated system

KW - Circulating tumor cells

KW - Lung cancer

KW - Whole blood

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JO - Biosensors and Bioelectronics

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ER -

Development of the automated circulating tumor cell recovery system with microcavity array

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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