TY - JOUR
T1 - Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum
AU - Ito, Etsuro
AU - Miyazawa, Atsuo
AU - Takagi, Hiroshi
AU - Yoshioka, Tohru
AU - Horikoshi, Tetsuro
AU - Yanagisawa, Keiji
AU - Nakamura, Takeshi
AU - Kudo, Yoshihisa
AU - Umeda, Masato
AU - Inoue, Keizo
AU - Mikoshiba, Katsuhiko
N1 - Funding Information:
We would like to thank Drs. J. de Barry, M.J. Berridge and R.F. Irvine for their comments on the manuscript and for many helpful discussions. This work was supported by a Grant-in-Aid (02241101) from the Japanese Ministry of Education, Science and Culture.
PY - 1991/8
Y1 - 1991/8
N2 - The postnatal development of calcium-mobilizing systems was studied by both microfluorometric imaging analysis of Ca2+ on living rat cerebellar slices and immunohistochemical labeling of phosphatidylinositol 4,5-bisphosphate (PIP2) and inositol 1,4,5-trisphosphate binding protein (IP3BP) in fixed rat cerebellum. Stimulation with quisqualate (QA) or N-methyl-d-aspartate (NMDA) enhanced the Ca2+ level only diffusely on postnatal day (PND) 3, but more discretely on PNDs 7 and 15. On PND 21, QA-induced responses were localized in the molecular layer especially, but not in the granular layer. By contrast, NMDA mobilized Ca2+ prominently in the granular layer, but only weakly in the molecular layer. Localized expression of PIP2 in the molecular layer paralleled QA-induced Ca2+ mobilization, but IP3BP was expressed more diffusely. The present study offers the first direct evidence that PIP2, but not IP3BP, is essential for QA-induced Ca2+ mobilization in the cerebellar cortex.
AB - The postnatal development of calcium-mobilizing systems was studied by both microfluorometric imaging analysis of Ca2+ on living rat cerebellar slices and immunohistochemical labeling of phosphatidylinositol 4,5-bisphosphate (PIP2) and inositol 1,4,5-trisphosphate binding protein (IP3BP) in fixed rat cerebellum. Stimulation with quisqualate (QA) or N-methyl-d-aspartate (NMDA) enhanced the Ca2+ level only diffusely on postnatal day (PND) 3, but more discretely on PNDs 7 and 15. On PND 21, QA-induced responses were localized in the molecular layer especially, but not in the granular layer. By contrast, NMDA mobilized Ca2+ prominently in the granular layer, but only weakly in the molecular layer. Localized expression of PIP2 in the molecular layer paralleled QA-induced Ca2+ mobilization, but IP3BP was expressed more diffusely. The present study offers the first direct evidence that PIP2, but not IP3BP, is essential for QA-induced Ca2+ mobilization in the cerebellar cortex.
KW - Cerebellar slice
KW - Development
KW - Excitatory amino acid
KW - Immunohistochemistry
KW - Inositol 1,4,5-trisphosphate binding protein
KW - Inositol phospholipid turnover
KW - Intracellular calcium concentration
KW - Phosphatidylinositol 4,5-bisphosphate
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U2 - 10.1016/0168-0102(91)90041-V
DO - 10.1016/0168-0102(91)90041-V
M3 - Article
C2 - 1661869
AN - SCOPUS:0025771454
SN - 0168-0102
VL - 11
SP - 179
EP - 188
JO - Neuroscience Research
JF - Neuroscience Research
IS - 3
ER -