TY - JOUR
T1 - Direct intracellular detection of biomolecule specific bound-water with Raman spectroscopy
AU - Samuel, Ashok Zachariah
AU - Sugiyama, Kaori
AU - Takeyama, Haruko
N1 - Funding Information:
The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Haruko Takeyama reports financial support was provided by Japan Society for the Promotion of Science.
Funding Information:
This work was supported by JSPS KAKENHI (Grant Number JP17H06158). KS acknowledges Life Science Center for Survival Dynamics, University of Tsukuba, Japan, for cell culture facility.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2023/1/15
Y1 - 2023/1/15
N2 - Lipids, proteins, and nucleic acids have closely associated water molecules (Bound water), which exhibit considerably different physical properties compared to bulk water. Here we investigate the possibility of resolving Raman spectra of the specific hydration shell of these biomolecules in intracellular regions using Raman imaging. Lipids and proteins + nucleic acids Raman spectral components resolved in the analysis showed associated water spectral features, which are uniquely different from that of bulk water. These spectral profiles agree with water spectral profile observed in the case of corresponding hydrated pure biomolecules. The results show the prospects of Raman imaging in examining intracellular hydration in biomolecules and its functional relation.
AB - Lipids, proteins, and nucleic acids have closely associated water molecules (Bound water), which exhibit considerably different physical properties compared to bulk water. Here we investigate the possibility of resolving Raman spectra of the specific hydration shell of these biomolecules in intracellular regions using Raman imaging. Lipids and proteins + nucleic acids Raman spectral components resolved in the analysis showed associated water spectral features, which are uniquely different from that of bulk water. These spectral profiles agree with water spectral profile observed in the case of corresponding hydrated pure biomolecules. The results show the prospects of Raman imaging in examining intracellular hydration in biomolecules and its functional relation.
KW - Biomolecule correlated water
KW - Intracellular hydration
KW - Multivariate curve resolution
KW - Raman imaging
KW - Single cell imaging
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U2 - 10.1016/j.saa.2022.121870
DO - 10.1016/j.saa.2022.121870
M3 - Article
C2 - 36116410
AN - SCOPUS:85138057801
SN - 1386-1425
VL - 285
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
M1 - 121870
ER -