Discovery of a pimaricin analog JBIR-13, from Streptomyces bicolor NBRC 12746 as predicted by sequence analysis of type i polyketide synthase gene

Hisayuki Komaki; Miho Izumikawa; Jun Ya Ueda; Takuji Nakashima; Shams Tabrez Khan; Motoki Takagi; Kazuo Shin-Ya

doi:10.1007/s00253-008-1849-8

Discovery of a pimaricin analog JBIR-13, from Streptomyces bicolor NBRC 12746 as predicted by sequence analysis of type i polyketide synthase gene

Hisayuki Komaki^*, Miho Izumikawa, Jun Ya Ueda, Takuji Nakashima, Shams Tabrez Khan, Motoki Takagi, Kazuo Shin-Ya

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Sequence analysis of ketosynthase domain amplicons from Streptomyces bicolor NBRC 12746^T revealed the presence of previously unreported type I polyketide synthases (PKS-I) genes. The clustering of these genes with the reference PKS-1 sequences suggested the possibility to produce a polyene compound similar to pimaricin. Thus, the cultured sample from NBRC 12746 ^T was analyzed for the production of polyene compounds. The strain produced an antifungal compound which displayed the UV absorption spectrum of tetraene macrolides. The structure determination based on the spectroscopic analysis of the purified compound resulted in the identification of a novel pimaricin analog JBIR-13 (1). This study therefore strongly suggested that a careful analysis of PKS-I genes can provide valuable information in the search of novel bioactive compounds within a class predicted from phylogenetic analysis.

Original language	English
Pages (from-to)	127-133
Number of pages	7
Journal	Applied Microbiology and Biotechnology
Volume	83
Issue number	1
DOIs	https://doi.org/10.1007/s00253-008-1849-8
Publication status	Published - 2009 May
Externally published	Yes

Keywords

JBIR-13
Pimaricin
Polyene macrolide
Streptomyces bicolor
Type I polyketide synthase

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

Access to Document

10.1007/s00253-008-1849-8

Cite this

@article{a21ff79e268f4e5287e95203f814db07,

title = "Discovery of a pimaricin analog JBIR-13, from Streptomyces bicolor NBRC 12746 as predicted by sequence analysis of type i polyketide synthase gene",

abstract = "Sequence analysis of ketosynthase domain amplicons from Streptomyces bicolor NBRC 12746T revealed the presence of previously unreported type I polyketide synthases (PKS-I) genes. The clustering of these genes with the reference PKS-1 sequences suggested the possibility to produce a polyene compound similar to pimaricin. Thus, the cultured sample from NBRC 12746 T was analyzed for the production of polyene compounds. The strain produced an antifungal compound which displayed the UV absorption spectrum of tetraene macrolides. The structure determination based on the spectroscopic analysis of the purified compound resulted in the identification of a novel pimaricin analog JBIR-13 (1). This study therefore strongly suggested that a careful analysis of PKS-I genes can provide valuable information in the search of novel bioactive compounds within a class predicted from phylogenetic analysis.",

keywords = "JBIR-13, Pimaricin, Polyene macrolide, Streptomyces bicolor, Type I polyketide synthase",

author = "Hisayuki Komaki and Miho Izumikawa and Ueda, {Jun Ya} and Takuji Nakashima and Khan, {Shams Tabrez} and Motoki Takagi and Kazuo Shin-Ya",

note = "Funding Information: Acknowledgments We are grateful to Dr. Tetsuo Suemoto, Ms. Natsumi Kuwahara, and Ms. Rieko Suzuki for their technical assistance. This work was supported by the grant from the New Energy and Industrial Technology Development Organization (NEDO) of Japan.",

year = "2009",

month = may,

doi = "10.1007/s00253-008-1849-8",

language = "English",

volume = "83",

pages = "127--133",

journal = "Applied Microbiology and Biotechnology",

issn = "0175-7598",

publisher = "Springer Verlag",

number = "1",

}

TY - JOUR

T1 - Discovery of a pimaricin analog JBIR-13, from Streptomyces bicolor NBRC 12746 as predicted by sequence analysis of type i polyketide synthase gene

AU - Komaki, Hisayuki

AU - Izumikawa, Miho

AU - Ueda, Jun Ya

AU - Nakashima, Takuji

AU - Khan, Shams Tabrez

AU - Takagi, Motoki

AU - Shin-Ya, Kazuo

N1 - Funding Information: Acknowledgments We are grateful to Dr. Tetsuo Suemoto, Ms. Natsumi Kuwahara, and Ms. Rieko Suzuki for their technical assistance. This work was supported by the grant from the New Energy and Industrial Technology Development Organization (NEDO) of Japan.

PY - 2009/5

Y1 - 2009/5

N2 - Sequence analysis of ketosynthase domain amplicons from Streptomyces bicolor NBRC 12746T revealed the presence of previously unreported type I polyketide synthases (PKS-I) genes. The clustering of these genes with the reference PKS-1 sequences suggested the possibility to produce a polyene compound similar to pimaricin. Thus, the cultured sample from NBRC 12746 T was analyzed for the production of polyene compounds. The strain produced an antifungal compound which displayed the UV absorption spectrum of tetraene macrolides. The structure determination based on the spectroscopic analysis of the purified compound resulted in the identification of a novel pimaricin analog JBIR-13 (1). This study therefore strongly suggested that a careful analysis of PKS-I genes can provide valuable information in the search of novel bioactive compounds within a class predicted from phylogenetic analysis.

AB - Sequence analysis of ketosynthase domain amplicons from Streptomyces bicolor NBRC 12746T revealed the presence of previously unreported type I polyketide synthases (PKS-I) genes. The clustering of these genes with the reference PKS-1 sequences suggested the possibility to produce a polyene compound similar to pimaricin. Thus, the cultured sample from NBRC 12746 T was analyzed for the production of polyene compounds. The strain produced an antifungal compound which displayed the UV absorption spectrum of tetraene macrolides. The structure determination based on the spectroscopic analysis of the purified compound resulted in the identification of a novel pimaricin analog JBIR-13 (1). This study therefore strongly suggested that a careful analysis of PKS-I genes can provide valuable information in the search of novel bioactive compounds within a class predicted from phylogenetic analysis.

KW - JBIR-13

KW - Pimaricin

KW - Polyene macrolide

KW - Streptomyces bicolor

KW - Type I polyketide synthase

UR - http://www.scopus.com/inward/record.url?scp=67349167956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349167956&partnerID=8YFLogxK

U2 - 10.1007/s00253-008-1849-8

DO - 10.1007/s00253-008-1849-8

M3 - Article

C2 - 19156407

AN - SCOPUS:67349167956

SN - 0175-7598

VL - 83

SP - 127

EP - 133

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 1

ER -

Discovery of a pimaricin analog JBIR-13, from Streptomyces bicolor NBRC 12746 as predicted by sequence analysis of type i polyketide synthase gene

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this