TY - JOUR
T1 - DNA methylation profiles provide a viable index for porcine pluripotent stem cells
AU - Arai, Yoshikazu
AU - Ohgane, Jun
AU - Fujishiro, Shuh Hei
AU - Nakano, Kazuaki
AU - Matsunari, Hitomi
AU - Watanabe, Masahito
AU - Umeyama, Kazuhiro
AU - Azuma, Dai
AU - Uchida, Naomi
AU - Sakamoto, Nozomu
AU - Makino, Tomohiro
AU - Yagi, Shintaro
AU - Shiota, Kunio
AU - Hanazono, Yutaka
AU - Nagashima, Hiroshi
PY - 2013/11
Y1 - 2013/11
N2 - Porcine induced pluripotent stem cells (iPSCs) provide useful information for translational research. The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor-intensive and costly process, necessitating a simple evaluation method for porcine iPSCs. Our previous study identified mouse embryonic stem cell (ESC)-specific hypomethylated loci (EShypo-T-DMRs), and, in this study, 36 genes selected from these were used to evaluate porcine iPSC lines. Based on the methylation profiles of the 36 genes, the iPSC line, Porco Rosso-4, was found closest to mouse pluripotent stem cells among 5 porcine iPSCs. Moreover, Porco Rosso-4 more efficiently contributed to the inner cell mass (ICM) of blastocysts than the iPSC line showing the lowest reprogramming of the 36 genes (Porco Rosso-622-14), indicating that the DNA methylation profile correlates with efficiency of ICM contribution. Furthermore, factors known to enhance iPSC quality (serum-free medium with PD0325901 and CHIR99021) improved the methylation status at the 36 genes. Thus, the DNA methylation profile of these 36 genes is a viable index for evaluation of porcine iPSCs. genesis 51:763-776.
AB - Porcine induced pluripotent stem cells (iPSCs) provide useful information for translational research. The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor-intensive and costly process, necessitating a simple evaluation method for porcine iPSCs. Our previous study identified mouse embryonic stem cell (ESC)-specific hypomethylated loci (EShypo-T-DMRs), and, in this study, 36 genes selected from these were used to evaluate porcine iPSC lines. Based on the methylation profiles of the 36 genes, the iPSC line, Porco Rosso-4, was found closest to mouse pluripotent stem cells among 5 porcine iPSCs. Moreover, Porco Rosso-4 more efficiently contributed to the inner cell mass (ICM) of blastocysts than the iPSC line showing the lowest reprogramming of the 36 genes (Porco Rosso-622-14), indicating that the DNA methylation profile correlates with efficiency of ICM contribution. Furthermore, factors known to enhance iPSC quality (serum-free medium with PD0325901 and CHIR99021) improved the methylation status at the 36 genes. Thus, the DNA methylation profile of these 36 genes is a viable index for evaluation of porcine iPSCs. genesis 51:763-776.
KW - Epigenetics
KW - Induced pluripotent stem cells
KW - Translational research
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U2 - 10.1002/dvg.22423
DO - 10.1002/dvg.22423
M3 - Article
C2 - 23913699
AN - SCOPUS:84888127352
SN - 1526-954X
VL - 51
SP - 763
EP - 776
JO - Genesis
JF - Genesis
IS - 11
ER -