TY - JOUR
T1 - DNA microarray mediated transcriptional profiling of Nitrosomonas europaea in response to linear alkylbenzene sulfonates
AU - Urakawa, Hidetoshi
AU - Matsumoto, Junpei
AU - Inaba, Kazuho
AU - Tsuneda, Satoshi
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/5
Y1 - 2008/5
N2 - Linear alkylbenzene sulfonates (LAS) constitute, quantitatively, the most important group of synthetic surfactants used today. We studied the gene expression of Nitrosomonas europaea in response to LAS using a DNA microarray because ammonia-oxidizers are thought to be more sensitive to LAS than other microorganisms. Our objective was to elucidate which genes are expressed for N. europaea in response to LAS exposure. Microarray analysis and real-time PCR assay revealed that c. 30 genes were significantly expressed after LAS exposure, in particular genes associated with energy production and conversion. Our findings demonstrate that physical disruption of membrane structures, which contain enzymes associated with energy production and conversion, might be an important explanation for the high sensitivity of N. europaea to LAS exposure.
AB - Linear alkylbenzene sulfonates (LAS) constitute, quantitatively, the most important group of synthetic surfactants used today. We studied the gene expression of Nitrosomonas europaea in response to LAS using a DNA microarray because ammonia-oxidizers are thought to be more sensitive to LAS than other microorganisms. Our objective was to elucidate which genes are expressed for N. europaea in response to LAS exposure. Microarray analysis and real-time PCR assay revealed that c. 30 genes were significantly expressed after LAS exposure, in particular genes associated with energy production and conversion. Our findings demonstrate that physical disruption of membrane structures, which contain enzymes associated with energy production and conversion, might be an important explanation for the high sensitivity of N. europaea to LAS exposure.
KW - Ammonia-oxidizing bacteria (AOB)
KW - Anionic detergent
KW - DNA microarrays
KW - Linear alkylbenzene sulfonates (LAS)
KW - Nitrosomonas europaea
KW - Surfactants
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U2 - 10.1111/j.1574-6968.2008.01111.x
DO - 10.1111/j.1574-6968.2008.01111.x
M3 - Article
C2 - 18371069
AN - SCOPUS:42449107250
SN - 0378-1097
VL - 282
SP - 166
EP - 173
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -