Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils

Qiang Liu; Katsuhiko Suzuki; Satoru Kudo; Mutsuo Yamada; Kenji Kowatari; Takashi Umeda; Shigeyuki Nakaji; Kazuo Sugawara

doi:10.1002/(SICI)1522-7243(199911/12)14:6<327::AID-BIO571>3.0.CO;2-A

Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils

Qiang Liu^*, Katsuhiko Suzuki, Satoru Kudo, Mutsuo Yamada, Kenji Kowatari, Takashi Umeda, Shigeyuki Nakaji, Kazuo Sugawara

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Decaglycerol mono-oleate (DGMO), a type of food additive (a polyglycerol ester of fatty acids), was evaluated for its in vitro effect on reactive oxygen species (ROS) generation by human neutrophils using a luminol-dependent chemiluminescence assay. Neutrophils were isolated by the Histopaque-1077/1119 double density gradient technique. Opsonized zymosan (OZ) and phorbol 12-myristate 13-acetate (PMA) were employed as artificial stimuli. DGMO inhibited the chemiluminescence response of OZ-stimulated neutrophils by 58% (p < 0.01) at a concentration of 0.5 mg/mL. DGMO inhibited the PMA-induced chemiluminescence by 40% (p < 0.01) at a concentration of 0.01 mg/mL. The inhibition phenomena could not be attributed to light absorption by DGMO alone, because the percentage chemiluminescence in the presence of DGMO is lower than the percentage transmittance of DGMO at 425 nm. These results suggest that DGMO downregulates ROS generation by human neutrophils.

Original language	English
Pages (from-to)	327-330
Number of pages	4
Journal	Luminescence
Volume	14
Issue number	6
DOIs	https://doi.org/10.1002/(SICI)1522-7243(199911/12)14:6<327::AID-BIO571>3.0.CO;2-A
Publication status	Published - 1999
Externally published	Yes

Keywords

Chemiluminescence
Decaglycerol mono-oleate
Human neutrophil
Reactive oxygen species

ASJC Scopus subject areas

Biophysics
Chemistry (miscellaneous)

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10.1002/(SICI)1522-7243(199911/12)14:6<327::AID-BIO571>3.0.CO;2-A

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title = "Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils",

abstract = "Decaglycerol mono-oleate (DGMO), a type of food additive (a polyglycerol ester of fatty acids), was evaluated for its in vitro effect on reactive oxygen species (ROS) generation by human neutrophils using a luminol-dependent chemiluminescence assay. Neutrophils were isolated by the Histopaque-1077/1119 double density gradient technique. Opsonized zymosan (OZ) and phorbol 12-myristate 13-acetate (PMA) were employed as artificial stimuli. DGMO inhibited the chemiluminescence response of OZ-stimulated neutrophils by 58% (p < 0.01) at a concentration of 0.5 mg/mL. DGMO inhibited the PMA-induced chemiluminescence by 40% (p < 0.01) at a concentration of 0.01 mg/mL. The inhibition phenomena could not be attributed to light absorption by DGMO alone, because the percentage chemiluminescence in the presence of DGMO is lower than the percentage transmittance of DGMO at 425 nm. These results suggest that DGMO downregulates ROS generation by human neutrophils.",

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author = "Qiang Liu and Katsuhiko Suzuki and Satoru Kudo and Mutsuo Yamada and Kenji Kowatari and Takashi Umeda and Shigeyuki Nakaji and Kazuo Sugawara",

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AU - Liu, Qiang

AU - Suzuki, Katsuhiko

AU - Kudo, Satoru

AU - Yamada, Mutsuo

AU - Kowatari, Kenji

AU - Umeda, Takashi

AU - Nakaji, Shigeyuki

AU - Sugawara, Kazuo

PY - 1999

Y1 - 1999

N2 - Decaglycerol mono-oleate (DGMO), a type of food additive (a polyglycerol ester of fatty acids), was evaluated for its in vitro effect on reactive oxygen species (ROS) generation by human neutrophils using a luminol-dependent chemiluminescence assay. Neutrophils were isolated by the Histopaque-1077/1119 double density gradient technique. Opsonized zymosan (OZ) and phorbol 12-myristate 13-acetate (PMA) were employed as artificial stimuli. DGMO inhibited the chemiluminescence response of OZ-stimulated neutrophils by 58% (p < 0.01) at a concentration of 0.5 mg/mL. DGMO inhibited the PMA-induced chemiluminescence by 40% (p < 0.01) at a concentration of 0.01 mg/mL. The inhibition phenomena could not be attributed to light absorption by DGMO alone, because the percentage chemiluminescence in the presence of DGMO is lower than the percentage transmittance of DGMO at 425 nm. These results suggest that DGMO downregulates ROS generation by human neutrophils.

AB - Decaglycerol mono-oleate (DGMO), a type of food additive (a polyglycerol ester of fatty acids), was evaluated for its in vitro effect on reactive oxygen species (ROS) generation by human neutrophils using a luminol-dependent chemiluminescence assay. Neutrophils were isolated by the Histopaque-1077/1119 double density gradient technique. Opsonized zymosan (OZ) and phorbol 12-myristate 13-acetate (PMA) were employed as artificial stimuli. DGMO inhibited the chemiluminescence response of OZ-stimulated neutrophils by 58% (p < 0.01) at a concentration of 0.5 mg/mL. DGMO inhibited the PMA-induced chemiluminescence by 40% (p < 0.01) at a concentration of 0.01 mg/mL. The inhibition phenomena could not be attributed to light absorption by DGMO alone, because the percentage chemiluminescence in the presence of DGMO is lower than the percentage transmittance of DGMO at 425 nm. These results suggest that DGMO downregulates ROS generation by human neutrophils.

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KW - Decaglycerol mono-oleate

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KW - Reactive oxygen species

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Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils

Abstract

Keywords

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