TY - JOUR
T1 - Effect of human serum on the electrical detection of amyloid-β fibrils in biological environments using azo-dye immobilized field effect transistor (FET) biosensor
AU - Hideshima, Sho
AU - Wustoni, Shofarul
AU - Kobayashi, Masumi
AU - Hayashi, Hiroki
AU - Kuroiwa, Shigeki
AU - Nakanishi, Takuya
AU - Osaka, Tetsuya
N1 - Funding Information:
This work is partly supported by the Center of Innovation Program, Building of Consortia for the Development of Human Resources in Science and Technology, and Grant-in-Aid for Young Scientists (B), all from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.
Publisher Copyright:
© 2018 The Authors
PY - 2018/2
Y1 - 2018/2
N2 - As amyloid-β peptide 1–42 (Aβ42) was found to be an emerging and important biomarker for Alzheimer's disease, the detection of this peptide in biological samples such as human serum (HS) has become very important for evaluating the potential disease state and determining the appropriate treatment. In this study, we developed an electrical analysis strategy based on a field effect transistor (FET) biosensor as a simple and reliable technique for confirming the presence of Aβ42 aggregates (fibrils) in biological samples. By utilizing Congo red immobilized on the FET gate surface as a biorecognition element, we observed remarkable sensitivity and specificity for detecting Aβ42 fibrils. Furthermore, we optimized the procedure to minimize the interference of abundant human serum albumin for the detection system using HS samples. The optimized system of Congo red-immobilized FET enables measurement of Aβ42 fibrils in the 100-pM level in HS samples, which is lower than its clinical concentration. The FET device can be applied as a biosensing system for mass and routine screening of peptide biomarkers related to Alzheimer's disease.
AB - As amyloid-β peptide 1–42 (Aβ42) was found to be an emerging and important biomarker for Alzheimer's disease, the detection of this peptide in biological samples such as human serum (HS) has become very important for evaluating the potential disease state and determining the appropriate treatment. In this study, we developed an electrical analysis strategy based on a field effect transistor (FET) biosensor as a simple and reliable technique for confirming the presence of Aβ42 aggregates (fibrils) in biological samples. By utilizing Congo red immobilized on the FET gate surface as a biorecognition element, we observed remarkable sensitivity and specificity for detecting Aβ42 fibrils. Furthermore, we optimized the procedure to minimize the interference of abundant human serum albumin for the detection system using HS samples. The optimized system of Congo red-immobilized FET enables measurement of Aβ42 fibrils in the 100-pM level in HS samples, which is lower than its clinical concentration. The FET device can be applied as a biosensing system for mass and routine screening of peptide biomarkers related to Alzheimer's disease.
KW - Albumin-amyloid beta complexes
KW - Alzheimer's disease
KW - Amyloid beta fibrils
KW - Field effect transistor biosensor
KW - Human serum albumin
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U2 - 10.1016/j.sbsr.2018.01.003
DO - 10.1016/j.sbsr.2018.01.003
M3 - Article
AN - SCOPUS:85044436623
SN - 2214-1804
VL - 17
SP - 25
EP - 29
JO - Sensing and Bio-Sensing Research
JF - Sensing and Bio-Sensing Research
ER -
