TY - JOUR
T1 - Effect of surface charge of magnetite nanoparticles on their internalization into breast cancer and umbilical vein endothelial cells
AU - Osaka, Tetsuya
AU - Nakanishi, Takuya
AU - Shanmugam, Sangaraju
AU - Takahama, Shintaro
AU - Zhang, Hong
N1 - Funding Information:
This work was financially supported in part by Special Coordination Funds for Promoting Science and Technology “Establishment of Consolidated Research Institute for Advanced Science and Medical Care” and Grant-in-Aid for Specially Promoted Research “Establishment of Electrochemical Device Engineering”, from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. SS acknowledges the Grant-in-Aid for JSPS Fellows, MEXT.
PY - 2009/7/1
Y1 - 2009/7/1
N2 - Internalization of magnetite nanoparticles with diameter of approximately 40 nm into normal and cancer cells was examined by microscopic observation and flow cytometry. Magnetite nanoparticles were synthesized by hydrolysis in an aqueous solution containing ferrous chloride with organic amines as a base. It was demonstrated that the difference in surface charge of magnetite nanoparticles brought about the difference in uptake efficiency. The nanoparticles with positive charge showed higher internalization into human breast cancer cells than the nanoparticles with negative charge, while the degree of internalization of the positively- and negatively-charged nanoparticles into human umbilical vein endothelial cells (HUVEC) was almost the same.
AB - Internalization of magnetite nanoparticles with diameter of approximately 40 nm into normal and cancer cells was examined by microscopic observation and flow cytometry. Magnetite nanoparticles were synthesized by hydrolysis in an aqueous solution containing ferrous chloride with organic amines as a base. It was demonstrated that the difference in surface charge of magnetite nanoparticles brought about the difference in uptake efficiency. The nanoparticles with positive charge showed higher internalization into human breast cancer cells than the nanoparticles with negative charge, while the degree of internalization of the positively- and negatively-charged nanoparticles into human umbilical vein endothelial cells (HUVEC) was almost the same.
KW - Cellular uptake
KW - Endocytosis
KW - Magnetite nanoparticles
KW - Surface charge
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U2 - 10.1016/j.colsurfb.2009.03.004
DO - 10.1016/j.colsurfb.2009.03.004
M3 - Article
C2 - 19361963
AN - SCOPUS:67349133115
SN - 0927-7765
VL - 71
SP - 325
EP - 330
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 2
ER -
