Effect of vitamin C depletion on age-related hearing loss in SMP30/GNL knockout mice

Akinori Kashio; Akiko Amano; Yoshitake Kondo; Takashi Sakamoto; Hitoshi Iwamura; Mitsuya Suzuki; Akihito Ishigami; Tatsuya Yamasoba

doi:10.1016/j.bbrc.2009.09.003

Effect of vitamin C depletion on age-related hearing loss in SMP30/GNL knockout mice

Akinori Kashio, Akiko Amano, Yoshitake Kondo, Takashi Sakamoto, Hitoshi Iwamura, Mitsuya Suzuki, Akihito Ishigami, Tatsuya Yamasoba^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Using senescence marker protein 30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), we examined whether modulating VC level affects age-related hearing loss (AHL). KO and wild-type (WT) C57BL/6 mice were given water containing 1.5 g/L VC [VC(+)] or 37.5 mg/L VC [VC(-)]. At 10 months of age, KO VC(-) mice showed significant reduction in VC level in the inner ear, plasma, and liver, increase in auditory brainstem response (ABR) thresholds, and decrease in the number of spiral ganglion cells compared to WT VC(-), WT VC(+), and KO VC(+) mice. There were no differences in VC level in the inner ear, ABR thresholds, or the number of spiral ganglion cells among WT VC(-), WT VC(+), and KO VC(+) mice. These findings suggest that VC depletion can accelerate AHL but that supplementing VC may not increase VC level in the inner ear or slow AHL in mice.

Original language	English
Pages (from-to)	394-398
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	390
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2009.09.003
Publication status	Published - 2009 Dec 18
Externally published	Yes

Keywords

Aging
Ascorbic acid
Cochlea
Gluconolactonase
Hearing loss
Oxidative stress
Senescence marker protein 30
Vitamin C

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2009.09.003

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title = "Effect of vitamin C depletion on age-related hearing loss in SMP30/GNL knockout mice",

abstract = "Using senescence marker protein 30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), we examined whether modulating VC level affects age-related hearing loss (AHL). KO and wild-type (WT) C57BL/6 mice were given water containing 1.5 g/L VC [VC(+)] or 37.5 mg/L VC [VC(-)]. At 10 months of age, KO VC(-) mice showed significant reduction in VC level in the inner ear, plasma, and liver, increase in auditory brainstem response (ABR) thresholds, and decrease in the number of spiral ganglion cells compared to WT VC(-), WT VC(+), and KO VC(+) mice. There were no differences in VC level in the inner ear, ABR thresholds, or the number of spiral ganglion cells among WT VC(-), WT VC(+), and KO VC(+) mice. These findings suggest that VC depletion can accelerate AHL but that supplementing VC may not increase VC level in the inner ear or slow AHL in mice.",

keywords = "Aging, Ascorbic acid, Cochlea, Gluconolactonase, Hearing loss, Oxidative stress, Senescence marker protein 30, Vitamin C",

author = "Akinori Kashio and Akiko Amano and Yoshitake Kondo and Takashi Sakamoto and Hitoshi Iwamura and Mitsuya Suzuki and Akihito Ishigami and Tatsuya Yamasoba",

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doi = "10.1016/j.bbrc.2009.09.003",

language = "English",

volume = "390",

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AU - Kashio, Akinori

AU - Amano, Akiko

AU - Kondo, Yoshitake

AU - Sakamoto, Takashi

AU - Iwamura, Hitoshi

AU - Suzuki, Mitsuya

AU - Ishigami, Akihito

AU - Yamasoba, Tatsuya

PY - 2009/12/18

Y1 - 2009/12/18

N2 - Using senescence marker protein 30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), we examined whether modulating VC level affects age-related hearing loss (AHL). KO and wild-type (WT) C57BL/6 mice were given water containing 1.5 g/L VC [VC(+)] or 37.5 mg/L VC [VC(-)]. At 10 months of age, KO VC(-) mice showed significant reduction in VC level in the inner ear, plasma, and liver, increase in auditory brainstem response (ABR) thresholds, and decrease in the number of spiral ganglion cells compared to WT VC(-), WT VC(+), and KO VC(+) mice. There were no differences in VC level in the inner ear, ABR thresholds, or the number of spiral ganglion cells among WT VC(-), WT VC(+), and KO VC(+) mice. These findings suggest that VC depletion can accelerate AHL but that supplementing VC may not increase VC level in the inner ear or slow AHL in mice.

AB - Using senescence marker protein 30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), we examined whether modulating VC level affects age-related hearing loss (AHL). KO and wild-type (WT) C57BL/6 mice were given water containing 1.5 g/L VC [VC(+)] or 37.5 mg/L VC [VC(-)]. At 10 months of age, KO VC(-) mice showed significant reduction in VC level in the inner ear, plasma, and liver, increase in auditory brainstem response (ABR) thresholds, and decrease in the number of spiral ganglion cells compared to WT VC(-), WT VC(+), and KO VC(+) mice. There were no differences in VC level in the inner ear, ABR thresholds, or the number of spiral ganglion cells among WT VC(-), WT VC(+), and KO VC(+) mice. These findings suggest that VC depletion can accelerate AHL but that supplementing VC may not increase VC level in the inner ear or slow AHL in mice.

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KW - Ascorbic acid

KW - Cochlea

KW - Gluconolactonase

KW - Hearing loss

KW - Oxidative stress

KW - Senescence marker protein 30

KW - Vitamin C

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Effect of vitamin C depletion on age-related hearing loss in SMP30/GNL knockout mice

Abstract

Keywords

ASJC Scopus subject areas

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